The m6A reader SlYTH2 negatively regulates tomato fruit aroma by impeding the translation process
文献类型： 外文期刊
作者： Bian, Hanxiao 1 ; Song, Peizhe 2 ; Gao, Ying 1 ; Deng, Zhiping 4 ; Huang, Chenyang 5 ; Yu, Lei 6 ; Wang, Hanqing 1 ; Ye, Bingbing 1 ; Cai, Zhihe 2 ; Pan, Yu 7 ; Wang, Fengqin 8 ; Liu, Jianzhao 5 ; Gao, Xiangwei 6 ; Chen, Kunsong 1 ; Jia, Guifang 2 ; Klee, Harry J. 1 ; Zhang, Bo 1 ;
作者机构： 1.Univ Fed Minas Gerais UFMG, Filosofia, Belo Horizonte, Brazil
2.Peking Univ, Coll Chem & Mol Engn, Synthet & Funct Biomol Ctr, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
3.Chongqing Univ, Sch Life Sci, Key Lab Plant Hormones & Dev Regulat Chongqing, Chongqing 401331, Peoples R China
4.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Peoples R China
5.Zhejiang Univ, Dept Polymer Sci & Engn, Key Lab Macromol Synth & Functionalizat, Minist Educ, Hangzhou 310058, Peoples R China
6.Zhejiang Univ, Sch Med, Sch Publ Hlth, Hangzhou 310058, Peoples R China
7.Southwest Univ, Key Lab Hort Sci Southern Mountainous Reg g, Minist Educ, Chongqing 400715, Peoples R China
8.Zhejiang Univ, Coll Anim Sci, Key Lab Anim Nutr & Feed Sci East China, Minist Agr, Hangzhou 310058, Peoples R China
9.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China
10.Peking Univ, Beijing Adv Ctr RNA Biol, Beijing 100871, Peoples R China
11.Zhejiang Univ, Hainan Inst, Sanya 572000, Hainan, Peoples R China
关键词： phase separation; translation repression; RNA methylation; flavor quality
期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：9.4； 五年影响因子：10.8 ）
ISSN： 0027-8424
年卷期： 2024 年 121 卷 28 期
页码：
收录情况： SCI
摘要： N 6- methyladenosine (m 6 A) is a fundamentally important RNA modification for gene regulation, whose function is achieved through m 6 A readers. However, whether and how m 6 A readers play regulatory roles during fruit ripening and quality formation remains unclear. Here, we characterized SlYTH2 as a tomato m 6 A reader protein and profiled the binding sites of SlYTH2 at the transcriptome- wide level. SlYTH2 undergoes liquid- liquid phase separation and promotes RNA-protein condensate formation. The target mRNAs of SlYTH2, namely m 6 A- modified SlHPL and SlCCD1B associated with volatile synthesis, are enriched in SlYTH2- induced condensates. Through polysome profiling assays and proteomic analysis, we demonstrate that knockout of SlYTH2 expedites the translation process of SlHPL and SlCCD1B , resulting in augmented production of aroma- associated volatiles. This aroma enrichment significantly increased consumer preferences for CRISPR- edited fruit over wild type. These findings shed light on the underlying mechanisms of m 6 A in plant RNA metabolism and provided a promising strategy to generate fruits that are more attractive to consumers.
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