文献类型： 外文期刊

作者： Bian, Hanxiao ¹ ; Song, Peizhe ² ; Gao, Ying ¹ ; Deng, Zhiping ⁴ ; Huang, Chenyang ⁵ ; Yu, Lei ⁶ ; Wang, Hanqing ¹ ; Ye, Bingbing ¹ ; Cai, Zhihe ² ; Pan, Yu ⁷ ; Wang, Fengqin ⁸ ; Liu, Jianzhao ⁵ ; Gao, Xiangwei ⁶ ; Chen, Kunsong ¹ ; Jia, Guifang ² ; Klee, Harry J. ¹ ; Zhang, Bo ¹ ;

作者机构： 1.Univ Fed Minas Gerais UFMG, Filosofia, Belo Horizonte, Brazil

2.Peking Univ, Coll Chem & Mol Engn, Synthet & Funct Biomol Ctr, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China

3.Chongqing Univ, Sch Life Sci, Key Lab Plant Hormones & Dev Regulat Chongqing, Chongqing 401331, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Peoples R China

5.Zhejiang Univ, Dept Polymer Sci & Engn, Key Lab Macromol Synth & Functionalizat, Minist Educ, Hangzhou 310058, Peoples R China

6.Zhejiang Univ, Sch Med, Sch Publ Hlth, Hangzhou 310058, Peoples R China

7.Southwest Univ, Key Lab Hort Sci Southern Mountainous Reg g, Minist Educ, Chongqing 400715, Peoples R China

8.Zhejiang Univ, Coll Anim Sci, Key Lab Anim Nutr & Feed Sci East China, Minist Agr, Hangzhou 310058, Peoples R China

9.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China

10.Peking Univ, Beijing Adv Ctr RNA Biol, Beijing 100871, Peoples R China

11.Zhejiang Univ, Hainan Inst, Sanya 572000, Hainan, Peoples R China

关键词： phase separation; translation repression; RNA methylation; flavor quality

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：9.4； 五年影响因子：10.8 ）

ISSN： 0027-8424

年卷期： 2024 年 121 卷 28 期

页码：

收录情况： SCI

摘要： N 6- methyladenosine (m 6 A) is a fundamentally important RNA modification for gene regulation, whose function is achieved through m 6 A readers. However, whether and how m 6 A readers play regulatory roles during fruit ripening and quality formation remains unclear. Here, we characterized SlYTH2 as a tomato m 6 A reader protein and profiled the binding sites of SlYTH2 at the transcriptome- wide level. SlYTH2 undergoes liquid- liquid phase separation and promotes RNA-protein condensate formation. The target mRNAs of SlYTH2, namely m 6 A- modified SlHPL and SlCCD1B associated with volatile synthesis, are enriched in SlYTH2- induced condensates. Through polysome profiling assays and proteomic analysis, we demonstrate that knockout of SlYTH2 expedites the translation process of SlHPL and SlCCD1B , resulting in augmented production of aroma- associated volatiles. This aroma enrichment significantly increased consumer preferences for CRISPR- edited fruit over wild type. These findings shed light on the underlying mechanisms of m 6 A in plant RNA metabolism and provided a promising strategy to generate fruits that are more attractive to consumers.