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The kiwifruit amyloplast proteome (kfALP): a resource to better understand the mechanisms underlying amyloplast biogenesis and differentiation

文献类型： 外文期刊

作者： Li, Ang 1 ; Lin, Jiajia 1 ; Zeng, Zhebin 1 ; Deng, Zhiping 2 ; Tan, Jinjuan 2 ; Chen, Xiaoya 1 ; Ding, Gang 1 ; Zhu, Man 1 ; Xu, Bin 1 ; Atkinson, Ross G. 3 ; Nieuwenhuizen, Niels J. 3 ; Ampomah-Dwamena, Charles 3 ; Cheng, Yunjiang 1 ; Deng, Xiuxin 1 ; Zeng, Yunliu 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry Sci, Natl R&D Ctr Citrus Preservat, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Wuhan 430070, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

3.New Zealand Inst Plant & Food Res Ltd PFR, Auckland 92169, New Zealand

关键词： Actinidia; amyloplast biogenesis; amyloplast differentiation; plastids; development; fruit quality; ripening

期刊名称：PLANT JOURNAL （ 影响因子：7.2； 五年影响因子：7.9 ）

ISSN： 0960-7412

年卷期： 2024 年 118 卷 2 期

页码：

收录情况： SCI

摘要： The biogenesis and differentiation (B&D) of amyloplasts contributes to fruit flavor and color. Here, remodeling of starch granules, thylakoids and plastoglobules was observed during development and ripening in two kiwifruit (Actinidia spp.) cultivars - yellow-fleshed 'Hort16A' and green-fleshed 'Hayward'. A protocol was developed to purify starch-containing plastids with a high degree of intactness, and amyloplast B&D was studied using label-free-based quantitative proteomic analyses in both cultivars. Over 3000 amyloplast-localized proteins were identified, of which >98% were quantified and defined as the kfALP (kiwifruit amyloplast proteome). The kfALP data were validated by Tandem-Mass-Tag (TMT) labeled proteomics in 'Hort16A'. Analysis of the proteomic data across development and ripening revealed: 1) a conserved increase in the abundance of proteins participating in starch synthesis/degradation during both amyloplast B & D; 2) up-regulation of proteins for chlorophyll degradation and of plastoglobule-localized proteins associated with chloroplast breakdown and plastoglobule formation during amyloplast differentiation; 3) constitutive expression of proteins involved in ATP supply and protein import during amyloplast B&D. Interestingly, two different pathways of amyloplast B&D were observed in the two cultivars. In 'Hayward', significant increases in abundance of photosynthetic- and tetrapyrrole metabolism-related proteins were observed, but the opposite trend was observed in 'Hort16A'. In conclusion, analysis of the kfALP provides new insights into the potential mechanisms underlying amyloplast B&D with relevance to key fruit quality traits in contrasting kiwifruit cultivars.

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