Two E-clade protein phosphatase 2Cs enhance ABA signaling by dephosphorylating ABI1 in Arabidopsis
文献类型： 外文期刊
作者： Zhang, Ya 1 ; Han, Liyuan 1 ; Liu, Junjie 1 ; Chang, Miao 1 ; Li, Chuanling 1 ; Shang, Jian-Xiu 1 ; Deng, Zhiping 2 ; Tang, Wenqiang 1 ; Sun, Yu 1 ;
作者机构： 1.Hebei Normal Univ, Hebei Collaborat Innovat Ctr Cell Signaling & Envi, Hebei Res Ctr Basic Discipline Cell Biol, Coll Life Sci,Key Lab Mol & Cellular Biol,Minist E, Shijiazhuang 050024, Hebei, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Peoples R China
3.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Key Lab Trop Fruit Biol, Minist Agr & Rural Affairs, Zhanjiang 524091, Peoples R China
关键词： abscisic acid signaling; ABI1; E-clade protein phosphatase 2C; dephosphorylation; ABA response
期刊名称：MOLECULAR PLANT （ 影响因子：24.1； 五年影响因子：25.8 ）
ISSN： 1674-2052
年卷期： 2025 年 18 卷 5 期
页码：
收录情况： SCI
摘要： ABA INSENSITIVE 1 (ABI1) and ABI2 are co-receptors of the phytohormone abscisic acid (ABA). Studies have demonstrated that phosphorylation of multiple amino acids on ABI1/2 augments their ability to inhibit ABA signaling in planta. However, whether and how the dephosphorylation of ABI1/2 is regulated to enhance plant sensitivity to ABA remain unknown. In this study, we identified two protein phosphatases, designated ABI1-Dephosphorylating E-clade PP2C 1 (ADEP1) and ADEP2, that interact with ABI1/2. Mutants lacking ADEP1, ADEP2, or both (adep1/2) exhibited reduced ABA inhibition of seed germination and root growth, as well as lower levels of ABA-induced stomatal closure. In addition, ABA-induced accumulation of ABI5 protein and expression of downstream target genes are reduced in the adep1/2 mutant compared with the wild type. These findings suggest that ADEP1/2 function as positive regulators of the ABA signaling pathway. Mass spectrometry analysis and two-dimensional electrophoresis identified Ser117 as a major ABA-induced phosphorylation site on the ABI1 protein. ADEP1/2 can dephosphorylate Ser117, leading to destabilization of the ABI1 protein and increased sensitivity of plants to ABA. Moreover, ABA treatment decreases the abundance of ADEP1/2 proteins. In summary, our study reveals two novel regulatory proteins that modulate ABA signaling and provides new insights into the regulatory network that fine-tunes plant ABA responses.
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