Prevalence and Multilocus Genotyping of Giardia duodenalis in Donkeys in Shanxi Province, North China
文献类型： 外文期刊
作者： Gao, Wen-Wei 1 ; Zhang, Shuo 1 ; Zhang, Tian-Hong 1 ; Xiao, Han-Dan 1 ; Su, Nan 1 ; Tao, Meng-Fan 2 ; Wu, Ze-Xuan 1 ; Zhang, Ze-Dong 1 ; Zhu, Xing-Quan 1 ; Xie, Shi-Chen 1 ;
作者机构： 1.Shanxi Agr Univ, Coll Vet Med, Lab Parasit Dis, Jinzhong 030801, Peoples R China
2.Shanxi Agr Univ, Coll Plant Protect, Shanxi Key Lab Integrated Pest Management Agr, Taiyuan 030031, Peoples R China
3.Yunnan Agr Univ, Coll Vet Med, Key Lab Vet Publ Hlth Higher Educ Yunnan Prov, Kunming 650201, Peoples R China
关键词： Giardia duodenalis; donkey; prevalence; assemblage; Shanxi Province
期刊名称：ANIMALS （ 影响因子：3.0； 五年影响因子：3.2 ）
ISSN： 2076-2615
年卷期： 2023 年 13 卷 24 期
页码：
收录情况： SCI
摘要： Giardia duodenalis is a ubiquitous flagellated protozoan, causing significant economic losses to animal husbandry and posing threats to public health. China ranks the world's sixth largest major producer of donkeys, rearing approximately 2.6 million donkeys in 2019, but limited investigation of G. duodenalis prevalence has been conducted in the past, and it is yet to be known whether donkeys in Shanxi Province are infected with G. duodenalis. In the present study, a total of 815 fecal samples collected from donkeys in representative regions of Shanxi Province, North China, were examined for G. duodenalis using nested PCR. Then, the assemblages and multilocus genotypes (MLGs) were examined based on three established loci: namely, beta-giardin (bg), triosephosphate isomerase (tpi), and glutamate dehydrogenase (gdh). The overall prevalence of G. duodenalis in donkeys in Shanxi Province was 16.81% (137/815). The region was identified as the main risk factor for the observed difference in G. duodenalis prevalence in donkeys among the three study areas (chi(2) = 21.611, p < 0.001). Assemblages A, E, and B were identified, with the latter as the predominant assemblage. Three MLGs (MLG-novel-1 to 3) were formed based on sequence variation among the three loci. The present study reveals the presence of G. duodenalis in donkeys in Shanxi Province, North China, for the first time, which not only enriches the data on the distribution of G. duodenalis in donkeys in China but also provides useful baseline data for planning control strategies against G. duodenalis infection in the sampled areas.
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