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Key Role of Heat Shock Protein Expression Induced by Ampicillin in Citrus Defense against Huanglongbing: A Transcriptomics Study

文献类型： 外文期刊

作者： Yang, Chuanyu 1 ; Powell, Charles 1 ; Duan, Yongping 3 ; Lin, Xiongjie 4 ; Fan, Goucheng 5 ; Hu, Hanqing 4 ; Zhang, Muqing 1 ;

作者机构： 1.Univ Florida, IRREC IFAS, 2199 S Rock Rd, Ft Pierce, FL 34945 USA

2.Texas A&M Univ Kingsville, Citrus Ctr, 312 N Int Blvd, Weslaco, TX 78579 USA

3.USDA, ARS, USHRL, 2011 S Rock Rd, Ft Pierce, FL 34945 USA

4.Fujian Acad Agr Sci, Inst Fruit Tree Res, Fuzhou 350003, Peoples R China

5.Fujian Acad Agr Sci, Inst Plant Protect, Fuzhou 350003, Peoples R China

6.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agri B, Nanning 530005, Peoples R China

关键词： Citrus Huanglongbing; ampicillin; plant defense; plant inducer; heat shock protein

期刊名称：AGRONOMY-BASEL （ 影响因子：3.949； 五年影响因子：4.117 ）

ISSN：

年卷期： 2022 年 12 卷 6 期

页码：

收录情况： SCI

摘要： Citrus Huanglongbing (HLB) is a serious disease for the citrus industry. Earlier studies showed that ampicillin (Amp) can reduce titers of the pathogen which causes HLB and the bacteria Candidatus Liberibacter asiaticus (CLas) in HLB-affected citrus. CLas has not yet been cultured, so the mechanisms of Amp against CLas are unclear. Some chemicals were demonstrated to trigger citrus defense systems against CLas. Therefore, we hypothesize that Amp may induce citrus defenses against CLas. Here we applied three nano-formulations of varying droplet sizes to HLB-affected citrus to achieve different accumulated concentrations of Amp (high, medium, low) in the plants. We then used RNA-seq to analyze induction of gene expression of citrus defense systems against CLas in response to different concentrations of Amp. The results indicated that at all accumulated concentrations of Amp can significantly suppress CLas titer and mitigate HLB symptoms. Transcriptomic analyses showed that Amp treatment induced expression of heat shock proteins (Hsps) in HLB-affected citrus, and these Hsps were significantly related to several defense genes encoding R proteins, transcription factors, splicing factors, RNA-binding proteins, RNA-dependent RNA polymerase, Gibberellic acid methyltransferase 2, L-ascorbate peroxidase 2, and ferruginol synthase that confer tolerance to CLas in citrus plants. Taken together, these results suggest that Amp treatment of citrus plants can trigger expression of Hsps and related defense genes to respond to CLas infection. These findings are valuable for developing novel strategies to combat citrus HLB.

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