The development and application of a LAMP assay for visualized detection of Chinese olive anthracnose
文献类型： 外文期刊
作者： Chen, Jin 1 ; Lan, Chengzhong 2 ; Hu, Hanqing 1 ; Lai, Ruilian 1 ; Wu, Rujian 1 ;
作者机构： 1.Fujian Acad Agr Sci, Fruit Res Inst, Fuzhou 350013, Peoples R China
2.Fujian Acad Agr Sci, Inst Plant Protect, Fuzhou 350013, Peoples R China
关键词： Chinese olive anthracnose; C; gloeosporioides; Loop-mediated isothermal amplification (LAMP); Translation elongation factor alpha 1 gene; Detection
期刊名称：JOURNAL OF PLANT DISEASES AND PROTECTION （ 影响因子：1.928； 五年影响因子：1.652 ）
ISSN： 1861-3829
年卷期： 2020 年 127 卷 4 期
页码：
收录情况： SCI
摘要： Purpose Anthracnose caused by Colletotrichum gloeosporioides (C. gloeosporioides) is an important disease affecting Chinese olive [Canarium album (Lour.) Rauesch] production. Therefore, developing a rapid and accurate method for C. gloeosporioides detection is of great importance for early diagnosis and immediate control of anthracnose. Method In the following study, we designed specific primers targeting the translation elongation factor alpha 1 gene of C. gloeosporioides, and established a loop-mediated isothermal amplification (LAMP) assay for fast and visualized detection system of C. gloeosporioides. The specificity and sensitivity of the LAMP assay were also measured. Results In the specificity test, characteristic bands were developed from 33 C. gloeosporioides isolates, and all of LAMP products showed green fluorescence after staining with SYBR Green I. On the other hand, negative results were obtained from C. gloeosporioides close species and other 19 common pathogens. The lower limit of LAMP detection was 0.4 fg center dot mu L-1. Conclusion This study provides a simple, highly sensitive, rapid and reliable technique for the detection of Chinese olive anthracnose.
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