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Molecular and functional characterization of a /3-tubulin gene in Plutella xylostella

文献类型： 外文期刊

作者： Liao, Jianying 1 ; Chen, Jing 1 ; Liu, Dan 1 ; Li, Jianwen 1 ; Chen, Jinzhi 6 ; Sun, Cuiying 1 ; Wei, Hui 7 ; Asad, Muhammad 1 ; Yang, Guang 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Inst Appl Ecol, State Key Lab Agr & Forestry Biosecur, Fuzhou 350002, Peoples R China

2.Minist Educ, Joint Int Res Lab Ecol Pest Control, Fuzhou 350002, Peoples R China

3.Fujian Agr & Forestry Univ, Ministerial & Prov Joint Innovat Ctr Safety Prod C, Fuzhou 350002, Peoples R China

4.Minist Agr, Key Lab Integrated Pest Management Fujian Taiwan C, Fuzhou 350002, Peoples R China

5.Fujian Prov Univ, Key Lab Green Pest Control, Fuzhou 350002, Peoples R China

6.Wenzhou Acad Agr Sci, Wenzhou Vocat Coll Sci & Technol, Southern Zhejiang Key Lab Crop Breeding, Wenzhou 325006, Peoples R China

7.Fujian Acad Agr Sci, Inst Plant Protect, Fuzhou 350013, Peoples R China

关键词： Diamondback moth; CRISPR/Cas9; Px/3tubulin-1; Reproductive fitness

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 300 卷

页码：

收录情况： SCI

摘要： The /3-tubulin gene is essential for reproductive development, especially for male fertility, in different insects including Bombyx mori and Drosophila melanogaster. Targeting reproductive genes such as /3-tubulin offers a promising approach to pest control that is more sustainable than chemical pesticides. However, there is limited research on the functional role of /3-tubulin in Plutella xylostella, a highly damaging pest of vegetable crops. In the present study, we first identified and cloned the /3-tubulin gene in P. xylostella (Px/3tubulin-1). Px beta tubulin-1 protein contains two conserved domains of Tubulin and Tubulin-C, and /3-tubulin were conserved in the Lepidoptera. Spatiotemporal expression analysis revealed that Px/3tubulin-1 was highly expressed in male pupae, adult males, and testes, suggesting its testis-specific function. Using CRISPR/Cas9 technology, we generated two homozygous Px/3tubulin-1 mutant strains of P. xylostella. Mutant strains exhibited significantly lower egg production and hatching rates compared with the wild type. Dissection and measurement of reproductive organs revealed that the testes and bursa copulatrix in mutant strains were significantly reduced in size compared with the wild type. In conclusion, Px/3tubulin-1 is vital for male fertility as it influences the development of reproductive organs and can be a potential target for the control of P. xylostella.

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