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Enhancing the Catalytic Activity of Type II L-Asparaginase from Bacillus licheniformis through Semi-Rational Design

文献类型： 外文期刊

作者： Zhou, Yawen 1 ; Jiao, Linshu 2 ; Shen, Juan 1 ; Chi, Huibing 1 ; Lu, Zhaoxin 1 ; Liu, Huawei 1 ; Lu, Fengxia 1 ; Zhu, Ping 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peoples R China

2.Jiangsu Acad Agr Sci, State Key Lab Breeding Base, Key Lab Food Qual & Safety Jiangsu Prov, Inst Food Safety & Nutr, Nanjing 210014, Peoples R China

关键词： L-asparaginase; protein engineering; saturation mutagenesis; catalytic efficiency; molecular dynamics simulation

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 17 期

页码：

收录情况： SCI

摘要： Low catalytic activity is a key factor limiting the widespread application of type II L-asparaginase (ASNase) in the food and pharmaceutical industries. In this study, smart libraries were constructed by semi-rational design to improve the catalytic activity of type II ASNase from Bacillus licheniformis. Mutants with greatly enhanced catalytic efficiency were screened by saturation mutations and combinatorial mutations. A quintuple mutant ILRAC was ultimately obtained with specific activity of 841.62 IU/mg and k(cat)/K-m of 537.15 min(-1)center dot mM(-1), which were 4.24-fold and 6.32-fold more than those of wild-type ASNase. The highest specific activity and k(cat)/K-m were firstly reported in type II ASNase from Bacillus licheniformis. Additionally, enhanced pH stability and superior thermostability were both achieved in mutant ILRAC. Meanwhile, structural alignment and molecular dynamic simulation demonstrated that high structure stability and strong substrate binding were beneficial for the improved thermal stability and enzymatic activity of mutant ILRAC. This is the first time that enzymatic activity of type II ASNase from Bacillus licheniformis has been enhanced by the semi-rational approach, and results provide new insights into enzymatic modification of L-asparaginase for industrial applications.

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