Precision SNP Genotyping for PVX Resistance Breeding in Potato: A Duplex Tetra-primer ARMS-PCR Approach Targeting the Rx1 and Rx2 Genes
文献类型： 外文期刊
作者： Islam, Saiful 1 ; Li, Jiana 1 ; Rahman, Mohammad Ataur 1 ; Song, Jiling 2 ; Xu, Binyan 1 ; Zheng, Jie 1 ; Zeng, Siyu 1 ; Song, Botao 1 ; Nie, Bihua 1 ;
作者机构： 1.Huazhong Agr Univ, Key Lab Potato Biol & Biotechnol, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Minist Agr & Rural Affairs, Wuhan 430070, Peoples R China
2.Heilongjiang Acad Agr Sci, Key Lab Potato Biol & Genet, Test Tube Plantlet Bank Natl Potato Germplasm Reso, Keshan Branch,Minist Agr & Rural Affairs, Qiqihar 161000, Peoples R China
关键词： Tetraploid Potato; Potato Virus X; Resistance Gene; SNP Genotyping; Tetra-primer ARMS-PCR
期刊名称：AMERICAN JOURNAL OF POTATO RESEARCH （ 影响因子：1.8； 五年影响因子：2.3 ）
ISSN： 1099-209X
年卷期： 2025 年
页码：
收录情况： SCI
摘要： The Rx1 and Rx2 genes from wild potato species confer extreme resistance to potato virus X (PVX). Accurate genotyping is essential for their integration into breeding programs. We developed tetra-primer ARMS-PCR markers targeting SNPs (G2515C in Rx1 and C2445G in Rx2) in the LRR regions to distinguish resistance alleles. These markers effectively genotyped 50 tetraploid potato varieties, identifying 16 as Rx1 heterozygous, 5 as Rx2 heterozygous, and 29 as homozygous susceptible consistent with phenotypic data. The T-ARMS-PCR outperformed existing markers and enabled efficient duplex detection of both genes. Although no homozygous resistant varieties were found, artificial DNA mixtures validated marker reliability for homozygous and heterozygous resistance detection. To our knowledge, this is the first use of T-ARMS-PCR for Rx1/Rx2 genotyping, offering a high-throughput tool for marker-assisted selection in developing PVX-resistant cultivars.
- 相关文献
作者其他论文 更多>>
-
GWAS combined with linkage analysis reveals major QTLs and candidate genes of salt tolerance in Japonica rice seedlings
作者：Xu, Shanbin;Zheng, Jie;Du, Haoqiang;Li, Chong;Duan, Yuxuan;Cai, Yanan;Wang, Jingguo;Liu, Hualong;Yang, Luomiao;Xin, Wei;Jia, Yan;Zou, Detang;Zheng, Hongliang;Du, Xiaodong
关键词：
Japonica rice seedling; salt tolerance; QTL; candidate gene; GWAS; linkage analysis -
Identifying Quantitative Trait Loci and Candidate Genes Conferring Resistance to Soybean Mosaic Virus SC7 by Quantitative Trait Loci-Sequencing in Soybean
作者：Zhang, Yong;Song, Jiling;Wang, Lei;Yang, Mengping;Hu, Kaifeng;Li, Weiwei;Sun, Xuhong;Xue, Hong;Dong, Quanzhong;Zhang, Mingming;Lou, Shubao;Yang, Xingyong;Du, Hao;Li, Yongli;Dong, Lidong;Cheng, Qun;Che, Zhijun
关键词：soybean; QTL; Soybean mosaic virus; SC7 strain; QTL-seq; MATE transporter
-
Genome assembly of primitive cultivated potato Solanum stenotomum provides insights into potato evolution
作者：Yan, Lang;Cai, Guangze;Qing, Yuan;Fang, Zhirong;Lai, Xianjun;Zhang, Yizheng;Wang, Haiyan;Tan, Xuemei;Song, Jiling;Liu, Chunsheng;Yang, Mengping;Lai, Xianjun
关键词：Solanum stenotomum; primitive cultivated potato; genome assembly; comparative genome; genomic variations
-
Geography-Driven Evolution of Potato Virus A Revealed by Genetic Diversity Analysis of the Complete Genome
作者：Zhang, Wei;Sun, Xuhong;Wei, Xuyan;Gao, Yanling;Song, Jiling;Bai, Yanju
关键词：potato virus A; genetic diversity; positive selection; phylogeny-trait association analysis; population structure
-
Genome assembly of primitive cultivated potato Solanum stenotomum provides insights into potato evolution
作者：Yan, Lang;Cai, Guangze;Qing, Yuan;Fang, Zhirong;Lai, Xianjun;Zhang, Yizheng;Wang, Haiyan;Tan, Xuemei;Song, Jiling;Liu, Chunsheng;Yang, Mengping
关键词：Solanum stenotomum; primitive cultivated potato; genome assembly; comparative genome; genomic variations
