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Application of culturomics in fungal isolation from mangrove sediments

文献类型： 外文期刊

作者： Li, Meng 1 ; Raza, Mubashar 1 ; Song, Shuang 1 ; Hou, Lingwei 4 ; Zhang, Zhi-Feng 5 ; Gao, Min 1 ; Huang, Jun-En 1 ; Liu, Fang 1 ; Cai, Lei 1 ;

作者机构： 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China

2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China

3.Xinjiang Acad Agr Sci, Inst Plant Protect, Key Lab Integrated Pest Management Crops Northwest, Minist Agr & Rural Affairs, Urumqi 830091, Peoples R China

4.China Astronaut Res & Training Ctr, Key Lab Space Nutr & Food Engn, Beijing 100094, Peoples R China

5.Southern Marine Sci & Engn Guangdong Lab Guangzhou, Guangzhou 511458, Peoples R China

关键词： Enrichment cultivation; In situ cultivation; Mangrove sediments; Rare dark matter fungi; Novel taxa

期刊名称：MICROBIOME （ 影响因子：15.5； 五年影响因子：19.4 ）

ISSN： 2049-2618

年卷期： 2023 年 11 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundFungi play a crucial role in ecosystems, and they have been widely considered a promising source for natural compounds that are crucial for drug discovery. Fungi have a high diversity, but about 95% of them remain unknown to science. The description rate of fungi is very low, mainly due to the inability of most fungi to grow in artificial media, which could not provide a sufficiently similar environment to their natural habitats. Moreover, many species in nature are in a state of low metabolic activity which cannot readily proliferate without proper resuscitation. Previously developed culturomics techniques are mostly designed and applicable for bacteria, with few attempts for fungal isolation because of their significantly larger cell size and hyphal growth properties.ResultsThis study attempted to isolate previously uncultured and rare fungi from mangrove sediments using newly developed fungal enrichment culture method (FECM) and fungal isolation chips (FiChips). Comparison of fungal community composition at different enrichment stages showed that FECM had great influence on fungal community composition, with rare taxa increased significantly, thus improving the isolation efficiency of previously uncultured fungi. Similarly, in situ cultivation using FiChips has a significant advantage in detecting and culturing rare fungi, as compared to the conventional dilution plate method (DPM). In addition, based on morphological comparisons and phylogenetic analyses, we described and proposed 38 new ascomycetous taxa, including three new families, eight new genera, 25 new species, and two new combinations (presented in additional file 1).ConclusionsOur study demonstrated that mangrove sediments harbor a high diversity of fungi, and our new isolation approaches (FECM and FiChips) presented a high efficiency in isolating hitherto uncultured fungi, which is potentially usable for fungal isolation in other similar environments.Dds2rK-5aaMj4bHfhLGEseVideo AbstractConclusionsOur study demonstrated that mangrove sediments harbor a high diversity of fungi, and our new isolation approaches (FECM and FiChips) presented a high efficiency in isolating hitherto uncultured fungi, which is potentially usable for fungal isolation in other similar environments.Dds2rK-5aaMj4bHfhLGEseVideo Abstract

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