文献类型： 外文期刊

作者： Zhang, Yuchen ¹ ; Plymale, Andrew ³ ; Son, Jiyoung ³ ; Huang, Qiaoyun ² ; Chen, Wenli ² ; Yu, Xiao-Ying ⁴ ;

作者机构： 1.Shanghai Acad Agr Sci, Natl Res Ctr Edible Fungi Biotechnol & Engn, Shanghai Key Lab Agr Genet & Breeding, Key Lab Appl Mycol Resources & Utilizat,Minist Agr, Shanghai, Peoples R China

2.Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan, Peoples R China

3.Pacific Northwest Natl Lab, Energy & Environm Directorate, Richland, WA USA

4.Oak Ridge Natl Lab, Mat Sci & Technol Div, Oak Ridge, TN 37830 USA

关键词： flow-cell culture; ToF-SIMS; biofilms; matrix effects; principal component analhysis (PCA)

期刊名称：FRONTIERS IN CHEMISTRY （ 影响因子：5.5； 五年影响因子：5.8 ）

ISSN： 2296-2646

年卷期： 2023 年 11 卷

页码：

收录情况： SCI

摘要： The interactions between soil microorganisms and soil minerals play a crucial role in the formation and evolution of minerals and the stability of soil aggregates. Due to the heterogeneity and diversity of the soil environment, the under-standing of the functions of bacterial biofilms in soil minerals at the microscale is limited. A soil mineral-bacterial biofilm system was used as a model in this study, and it was analyzed by time-of-flight secondary ion mass spectrometry (ToF-SIMS) to acquire molecular level information. Static culture in multi-wells and dynamic flow-cell culture in microfluidics of biofilms were investigated. Our results show that more characteristic molecules of biofilms can be observed in SIMS spectra of the flow-cell culture. In contrast, biofilm signature peaks are buried under the mineral components in SIMS spectra in the static culture case. Spectral overlay was used in peak selection prior to performing Principal component analysis (PCA). Comparisons of the PCA results between the static and flow-cell culture show more pronounced molecular features and higher loadings of organic peaks of the dynamic cultured specimens. For example, fatty acids secreted from bacterial biofilm extracellular polymeric substance are likely to be responsible for biofilm dispersal due to mineral treatment up to 48 h. Such findings suggest that the use of microfluidic cells to dynamically culture biofilms be a more suitable method for reducing the matrix effect arisen from the growth medium and minerals as a perturbation factor for improved spectral and multivariate analysis of complex mass spectral data in ToF-SIMS. These results show that the interaction mechanism between biofilms and soil minerals at the molecular level can be better studied using the flow-cell culture and advanced mass spectral imaging techniques like ToF-SIMS.