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An improved method to efficiently acquire rice black-streaked dwarf virus viruliferous small brown planthoppers

文献类型： 外文期刊

作者： Du, Linlin 1 ; Zeng, Bo 4 ; Li, Xuejuan 2 ; Lan, Ying 2 ; Guo, Wei 2 ; Wang, Zhaoyun 2 ; Liu, Zhiyang 2 ; Zhou, Yijun 2 ; Jiban, Kumar Kunda 5 ; Zhou, Tong 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Peoples R China

2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Quality & Safety, State Key Lab Cultivat Base, Minist Sci & Technol,Int Rice Res Inst, Nanjing, Peoples R China

3.Jiangsu Acad Agr Sci, Jiangsu Acad Agr Sci Joint Lab, Inst Plant Protect, Nanjing, Peoples R China

4.Natl Agr Technol Extens Serv Ctr, Beijing, Peoples R China

5.Crop Res Inst, Ctr Plant Virus Res, Plant Virus & Vector Interact, Prague, Czech Republic

关键词： RBSDV; viruliferous SBPHs; feeding times; RT-qPCR; virus loads

期刊名称：FRONTIERS IN GENETICS （ 影响因子：3.7； 五年影响因子：4.3 ）

ISSN：

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： Accurate phenotypic identification is the basis of research for resistance genetics and rice breeding for resistance to RBSDV disease. Obtaining rice black-streaked dwarf virus (RBSDV) viruliferous small brown planthoppers (SBPHs) with high transmission efficiency is an essential part of accurate phenotypic identification. Here, through quantifying number of RBSDV copies in infected rice plants, optimizing times of SBPHs fed on RBSDV-infected rice plants and leaf stage of rice seedlings, a method to acquire an RBSDV-carrying SBPH population more efficiently was improved. The results showed that rate of viruliferous SBPHs was significantly higher when fed on RBSDV-infected rice plants that had the copy numbers of RBSDV S10 of 3.0*10(4) and 1.1*10(4) than 8.3*10(2). Therefore, it is more efficient for SBPHs to acquire the virus when fed on RBSDV-infected rice plants that have copy numbers of RBSDV S10 above 1.1*10(4). The rate of viruliferous SBPHs were 50% and 54%, respectively, after the insects fed on RBSDV-infected rice plants for 7 and 9 days and being transferred to healthy rice seedlings for 5 and 3 days, which was significantly higher than those at other feeding times. The optimal inoculation leaf stage of rice seedlings was the 2-3-leaf stage (3 effective SBPHs per seedling for 72 h), but a high rate of viruliferous SBPHs may be suggested for inoculation of older rice seedlings.

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