Preparation of a cattle bone collagen peptide-calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells
文献类型： 外文期刊
作者： Zhang, Hongru 1 ; Qi, Liwei 1 ; Wang, Xiaodan 3 ; Guo, Yujie 1 ; Liu, Jiqian 1 ; Xu, Yang 5 ; Liu, Chengjiang 4 ; Zhang, Chunhui 1 ; Richel, Aurore 2 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Food Sci & Technol, Key Lab Agroprod Proc, Minist Agr & Rural Affairs, Beijing 100193, Peoples R China
2.Univ Liege, Lab Biomass & Green Technol, Gembloux Agrobio Tech, Passage Deportes 2, B-5030 Gembloux, Belgium
3.Wageningen Univ & Res, Dept Anim Sci, Adaptat Physiol Grp, NL-6708 WD Wageningen, Netherlands
4.Technol Xinjiang Acad Agr & Reclamat Sci, Inst Agroprod Proc Sci, Shihezi 832000, Peoples R China
5.Inner Mongolia Peptide Mengtai Biol Engn Co Ltd, Shengle Econ Pk, Hohhot 010000, Inner Mongolia, Peoples R China
期刊名称：FOOD & FUNCTION （ 影响因子：6.317； 五年影响因子：6.375 ）
ISSN： 2042-6496
年卷期：
页码：
收录情况： SCI
摘要： This study was designed to prepare a cattle bone-derived collagen peptide-calcium chelate by the ultrasound method (CP-Ca-US), and its structure, stability, and bioactivity on MC3T3-E1 cells were characterized. Single-factor experiments optimized the preparation conditions: ultrasound power 90 W, ultrasound time 40 min, CaCl2/peptides ratio 1/2, pH 7. Under these conditions, the calcium-chelating ability reached 39.48 mu g mg(-1). The result of Fourier transform-infrared spectroscopy indicated that carboxyl oxygen and amino nitrogen atoms were chelation sites. Morphological analysis indicated that CP-Ca-US was characterized by a porous surface and large particles. Stability analysis demonstrated that CP-Ca-US was stable in the thermal environment and under intestinal digestion. CP-Ca-US showed more stability in gastric juice than the chelate prepared by the hydrothermal method. Cell experiments indicated that CP-Ca-US increased osteoblast proliferation (proliferation rate 153% at a concentration of 300 mu g mL(-1)) and altered the cell cycle. Significantly, CP-Ca-US enhanced calcium absorption by interacting with calcium-sensing receptors and promoted the mineralization of MC3T3-E1 cells. This study provides the scientific basis for applying the ultrasound method to prepare peptide-calcium chelates and clarifies the positive role of chelates in bone building.
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