文献类型： 外文期刊

作者： Li, Zhimei ¹ ; Zhou, Hougao ¹ ; Chen, Yan ² ; Chen, Minyi ¹ ; Yao, Yutong ¹ ; Luo, Honghui ¹ ; Wu, Qing ¹ ; Wang, Fenglan ¹ ; Zhou, Yiwei ⁴ ;

作者机构： 1.Zhongkai Univ Agr & Engn, Coll Hort & Landscape Architecture, Guangzhou 510225, Peoples R China

2.Renshan Tianyuan Guangdong Agr Sci & Technol Dev C, Yunfu 527329, Peoples R China

3.Zhonghua Modern Agr Res Inst, Dept Gastroenterol, Guangzhou 510800, Peoples R China

4.Guangdong Acad Agr Sci, Environm Hort Res Inst, Guangdong Prov Key Lab Ornamental Plant Germplasm, Guangzhou 510640, Peoples R China

关键词： chrysanthemum; high temperature; anthocyanin; flower color; transcriptome

期刊名称：AGRONOMY-BASEL （ 影响因子：3.4； 五年影响因子：3.8 ）

ISSN：

年卷期： 2024 年 14 卷 12 期

页码：

收录情况： SCI

摘要： Flower color is one of the most important ornamental traits of chrysanthemums. Previous studies have shown that high temperatures can cause the petals of some chrysanthemum varieties to fade; however, the molecular mechanisms behind this phenomenon remain poorly understood. This study examines the mechanisms of color change in purple chrysanthemums under high-temperature stress using combined metabolomic and transcriptomic analyses. Four chrysanthemum varieties-two heat-stable ('Zi Feng Che' and 'Chrystal Regal') and two heat-sensitive ('Zi Hong Tuo Gui' and 'Zi Lian')-were analyzed. High-temperature conditions (35 degrees C) significantly downregulated key anthocyanins in heat-sensitive varieties, particularly cyanidin-3-O-(3 '',6 ''-O-dimalonyl)glucoside and pelargonidin-3-O-(3 '',6 ''-O-dimalonyl)glucoside. Transcriptome analysis revealed differential gene expression involved in anthocyanin biosynthesis and degradation, with significant enrichment in the MAPK signaling, phenylpropanoid biosynthesis, flavonoid biosynthesis, and anthocyanin biosynthesis pathways. The study highlighted the differential expression of CHS, DFR, ANS, GT1, 3AT, and UGT75C1 genes in anthocyanin synthesis between heat-sensitive and heat-tolerant varieties. Compared to heat-stable varieties, the petals of heat-sensitive varieties exhibited greater differential expression of heat-responsive transcription factors, including HSFs, ERFs, MYBs, and WRKYs. Genes that show a significant negative correlation with the downregulated anthocyanins, including Cse_sc012959.1_g030.1 (beta G), Cse_sc001798.1_g020.1 (MYB), Cse_sc006944.1_g010.1 (MYB), and Cse_sc000572.1_g090.1 (HSF), might regulate anthocyanin accumulation in chrysanthemums in response to high-temperature stress. These results provide guidance for the cultivation management and variety selection of chrysanthemums under high-temperature conditions. Additionally, they lay the foundation for elucidating the molecular mechanisms of flower color stability under heat stress and for breeding new heat-tolerant varieties.