文献类型： 外文期刊

作者： Shi, Chun-Hui ¹ ; Wang, Xiao-Qing ¹ ; Xu, Jian-Feng ² ; Zhang, Yu-Xing ² ; Qi, Baoxiu ² ; Jun, Luo ¹ ;

作者机构： 1.Shanghai Acad Agr Sci, Forest & Fruit Tree Res Inst, Shanghai 201403, Peoples R China

2.Hebei Agr Univ, Coll Hort, Baoding 071001, Hebei, Peoples R China

3.Liverpool John Moores Univ, Sch Pharm & Biomol Sci, James Parsons Bldg,Byrom St, Liverpool L3 3AF, Merseyside, England

4.Beijing Univ Agr, Beijing Adv Innovat Ctr Tree Breeding Mol Design, 7 Beinong Rd, Beijing, Peoples R China

关键词： sand pear; russeting; multi-omics; suberin; rainfall; PbHHT1

期刊名称：PLANT JOURNAL （ 影响因子：6.486； 五年影响因子：7.666 ）

ISSN： 0960-7412

年卷期：

页码：

收录情况： SCI

摘要： Brown coloration and a rough appearance as russet and semi-russet (partial russet) are features unique to the popular Asian sand pear (Pyrus pyrifolia Nakai). The degree of russeting is different between different genotypes. Russeting is sensitive to water fluctuations, where excessive rainwater can trigger/stimulate its development. However, the molecular mechanism of russeting is currently unclear. Here, we employed multi-omics, i.e., metabolomics, transcriptomics, and proteomics, and analyzed the effect of different sand pear genotypes and artificial rainfall on russeting of pear fruits. This led to the identification of 79, 64, and 29 differentially produced/expressed metabolites, transcripts, and proteins that are involved in the biosynthesis of suberin, phenylpropane, cutin, and waxes. Further analysis of these differentially expressed genes and their encoded proteins revealed that four of them exhibited high expression at both transcript and protein levels. Transient expression of one such gene, PbHHT1 (accession number 103966555), which encodes omega-hydroxypalmitate-O-feruloyl transferase, in young green non-russet fruits triggered premature suberization in the russeting pear genotypes. This coincided with increased production of 16-feruloyloxypalmitic acid, a conjugated compound between phenols and esters during the polymerization for suberin formation. Collectively, our data from the combined three omics demonstrate that russeting in sand pear is a complex process involving the biosynthesis and transport of suberin and many other secondary metabolites.