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Functional Characterization of Largemouth Bass (Micropterus salmoides) Soluble Fc gamma R Homolog in Response to Bacterial Infection

文献类型： 外文期刊

作者： Wu, Jing 1 ; Ma, Yanping 1 ; Nie, Yifan 1 ; Wang, Jingya 2 ; Feng, Guoqing 1 ; Hao, Le 1 ; Huang, Wen 5 ; Li, Yugu 2 ; Liu, Zhenxing 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Peoples R China

2.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Peoples R China

3.Key Lab Livestock Dis Prevent Guangdong Prov, Guangzhou 510640, Peoples R China

4.Minist Agr & Rural Affairs, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Guangzhou 510640, Peoples R China

5.Guangdong Acad Agr Sci, Collaborat Innovat Ctr, Guangzhou 510640, Peoples R China

6.Guangdong Acad Agr Sci, Inst Anim Sci, Guangdong Key Lab Anim Breeding & Nutr, Guangzhou 510640, Peoples R China

关键词： largemouth bass; soluble Fc gamma Rs; IgM-binding; ADCP; Nocardia seriolae

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 22 期

页码：

收录情况： SCI

摘要： Fc receptors (FcRs) are key players in antibody-dependent cellular phagocytosis (ADCP) with their specific recognition of the Fc portion of an immunoglobulin. Despite reports of Fc gamma R-mediated phagocytosis in mammals, little is known about the effects of soluble Fc gamma Rs on the immune response. In this study, Fc gamma RI alpha was cloned from the largemouth bass (Micropterus salmoides) (MsFc gamma RI alpha). Without a transmembrane segment or a cytoplasmic tail, MsFc gamma RI alpha was identified as a soluble form protein and widely distributed in the spleen, head kidney, and intestine. The native MsFc gamma RI alpha was detected in the serum of Nocardia seriolae-infected largemouth bass and the supernatants of transfected HEK293 cells. Additionally, it was verified that the transfected cells' surface secreted MsFcRI alpha could bind to largemouth bass IgM. Moreover, the expression changes of MsFc-gamma RI alpha, Syk, and Lyn indicated that MsFc-gamma RI alpha was engaged in the acute phase response to bacteria, and the Fc gamma R-mediated phagocytosis pathway was activated by Nocardia seriolae stimulation. Furthermore, recombinant MsFc gamma RI alpha could enhance both reactive oxygen species (ROS) and phagocytosis to Nocardia seriolae of leukocytes, presumably through the interaction of MsFc gamma RI alpha with a complement receptor. In conclusion, these findings provided a better understanding of the function of soluble Fc gamma Rs in the immune response and further shed light on the mechanism of phagocytosis in teleosts.

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