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Tandemly duplicated genes encoding polygalacturonase inhibitors are associated with bruchid (Callosobruchus chinensis) resistance in moth bean (Vigna aconitifolia)

文献类型： 外文期刊

作者： Gamage, Shyali Iroshani Rathnayaka 1 ; Kaewwongwal, Anochar 1 ; Laosatit, Kularb 1 ; Yimram, Tarika 1 ; Lin, Yun 3 ; Chen, Xin 3 ; Nakazono, Mikio 2 ; Somta, Prakit 1 ;

作者机构： 1.Kasetsart Univ, Fac Agr Kamphaeng Saen, Dept Agron, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand

2.Nagoya Univ, Grad Sch Bioagr Sci, Furo Cho,Chikusa Ku, Nagoya 4648601, Japan

3.Jiangsu Acad Agr Sci, Inst Ind Crops, Nanjing 210014, Peoples R China

关键词： Bruchid; Moth bean; Callosobruchus; PGIP; Polygalacturonase inhibitor; Polygalacturonase-inhibiting protein

期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）

ISSN： 0168-9452

年卷期： 2022 年 323 卷

页码：

收录情况： SCI

摘要： Bruchids are stored-grain insect pests responsible for serious seed loss in legume crops. A previous study using an F2 population (F2OA) derived from a cross between wild moth-bean (Vigna aconitifolia [Jacq.] Mare & PRIME;chal) accession TN67 (resistant) and cultivated moth-bean accession ICPMO056 (susceptible) revealed that resistance to the azuki bean weevil (Callosobruchus chinensis L.) in TN67 was regulated by a single gene located in the major quantitative trait locus-qVacBrc2.1. In this study, qVacBrc2.1 was finely mapped and candidate genes in this locus were identified using F2OA and another large F2 population (F2NB) derived from the cross mentioned previously. In contrast to the previous study, segregation analysis in the F2NB population revealed that resistance against this pest was controlled by two genes. Furthermore, the addition of novel markers to qVacBrc2.1 and reanalysis of the QTL in the F2OA population demonstrated that qVacBrc2.1 constituted two linked QTLs-qVacBrc2.1-A and qVacBrc2.1-B. The presence of qVacBrc2.1-B was verified using the population F2NB. Comparative genomics using three Vigna spp. strongly suggested the presence of two tandemly duplicated genes, VacPGIP1 and VacPGIP2, which encoded polygalacturonase inhibitors (polygalacturonase-inhibiting proteins) as the candidates for conferring resistance, but only VacPGIP1 could be successfully cloned and sequenced. The alignment of VacPGIP1 coding sequences of TN67 and ICPMO056 revealed eight single nucleotide poly-morphisms, three of which altered the amino-acid sequence of the predicted domains of polygalacturonase in-hibitors in ICPMO056. Overall, these findings indicate that VacPGIP1 and VacPGIP2 regulated C. chinensis resistance in TN67.

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