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A comparative authentication study of fresh fruit and vegetable juices using whole juice and sugar-specific stable isotopes

文献类型： 外文期刊

作者： Wu, Hao 1 ; Zhou, Xiuwen 1 ; Chen, Haiquan 1 ; Jin, Baohui 1 ; Yan, Zhi 1 ; Xie, Liqi 1 ; Zhou, Haichao 2 ; Rogers, Karyne M. 3 ; Lin, Guanghui 5 ;

作者机构： 1.Food Inspect & Quarantine Ctr, Shenzhen Customs, Shenzhen 518033, Peoples R China

2.Shenzhen Univ, Sch Life & Marine Sci, Shenzhen 518055, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Qual & Stand Agr Prod, Hangzhou 310021, Peoples R China

4.GNS Sci, Natl Isotope Ctr, Lower Hutt 5040, New Zealand

5.Tsinghua Univ, Grad Sch Shenzhen, Shenzhen 518055, Guangdong, Peoples R China

关键词： Fresh juice; Adulteration; Carbon and oxygen isotopes; CSIA stable isotope; HPLC-co-IRMS

期刊名称：FOOD CHEMISTRY （ 影响因子：9.231； 五年影响因子：8.795 ）

ISSN： 0308-8146

年卷期： 2022 年 373 卷

页码：

收录情况： SCI

摘要： Not-from-concentrate (NFC) juice has better nutrition, flavor and higher price than reconstituted juice. Accordingly, NFC juice is prone to adulteration and is an ongoing industry problem that has not yet been resolved. Undeclared addition of water and sugar are the main forms of NFC juice adulteration. This paper investigates the carbon and oxygen stable isotope ratios (delta C-13 and delta O-18 values) of the bulk juice and different juice components from 21 fruit and vegetable juices, and qualitatively and quantitatively analyzes the addition of water and sugar in NFC juices. The results show that the use of fruit pulp can help to qualitatively and quantitatively indicate the presence of C4 plant sugars in NFC juice, and can reliably detect added C4 plant sugars above 7 %. Sugar-specific isotope analysis (SSIA) technology was used to determine the delta C-13 values of different sugars (sucrose, glucose and fructose) and carbon content to qualitatively infer C3 plant sugar addition. Pulp extracted from juice had a good linear relationship with the juice water delta O-18 values (R-2 >0.90). The addition of water to NFC juice can also be determined by comparing delta O-18 values of extraneous water, pulp and filtered juice. Stable isotope technology confirmed NFC juice adulteration of in-market samples using the pulp as an internal reference and was found to be a useful tool to detect adulteration of in-market NFC juice.

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