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Rapid, specific and sensitive detection of Vibrio parahaemolyticus in seafood by accelerated strand exchange amplification

文献类型： 外文期刊

作者： Sun, Ritong 1 ; Chen, Jiao 1 ; Wang, Yingeng 2 ; Zhang, Zheng 2 ; Li, Yong 1 ; Li, Fengmei 1 ; Ma, Cuiping 1 ; Han, Qingxia 3 ; Shi, Yanjing 1 ;

作者机构： 1.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao Nucl Acid Rapid Detect Engn Res Ctr, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

3.Bin Zhou Polytech, Qingdao 256600, Peoples R China

期刊名称：ANALYTICAL METHODS （ 影响因子：3.1； 五年影响因子：3.0 ）

ISSN： 1759-9660

年卷期： 2023 年 15 卷 5 期

页码：

收录情况： SCI

摘要： Vibrio parahaemolyticus infectious diseases caused by seafood contamination may be life-threatening to people with weak immunity. The detection of the Vibrio parahaemolyticus pathogen in aquatic foods is critical for reducing the outbreak of human Vibrio parahaemolyticus-associated diseases. In this study, a highly sensitive, specific, and time-saving real-time narrow thermal-cycling amplification detection method was developed based on accelerated strand exchange amplification (ASEA). It can detect cultured Vibrio parahaemolyticus at concentrations as low as 25 CFU mL(-1). In addition, for artificially spiked scallop meat, the detection limit was 1.8 x 10(3) CFU g(-1) without pre-culture and 18 CFU g(-1) of initial inoculum after 3 h enrichment. The whole assay, starting from DNA extraction, can be completed within 20 min. The ASEA detection method established in this study is an effective tool for the rapid detection of Vibrio parahaemolyticus strains in a large number of seafood samples.

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