文献类型： 外文期刊

作者： Ma, Yinbo ¹ ; Choi, Su Ryun ¹ ; Wang, Yu ¹ ; Chhapekar, Sushil Satish ² ; Zhang, Xue ¹ ; Wang, Yingjun ¹ ; Zhang, Xueying ¹ ; Zhu, Meiyu ¹ ; Liu, Di ¹ ; Zuo, Zhennan ¹ ; Yan, Xinyu ¹ ; Gan, Caixia ³ ; Zhao, Di ⁴ ; Liang, Yue ⁵ ; Pang, Wenxing ¹ ; Lim, Yong Pyo ² ;

作者机构： 1.Shenyang Agr Univ, Coll Hort, Shenyang 110866, Peoples R China

2.Chungnam Natl Univ, Dept Hort, Mol Genet & Genom Lab, Daejeon 305764, South Korea

3.Hubei Acad Agr Sci, Cash Crops Res Inst, Hubei Key Lab Vegetable Germplasm Enhancement & G, Wuhan 430070, Peoples R China

4.Shenyang Agr Univ, Analyt & Testing Ctr, Shenyang 110866, Peoples R China

5.Shenyang Agr Univ, Coll Plant Protect, Shenyang 110866, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 影响因子：7.291； 五年影响因子：7.487 ）

ISSN： 2662-6810

年卷期： 2022 年 9 卷

页码：

收录情况： SCI

摘要： Clubroot is one of the major diseases adversely affecting Chinese cabbage (Brassica rapa) yield and quality. To precisely characterize the Plasmodiophora brassicae infection of Chinese cabbage, we developed a dual fluorescent staining method for simultaneously examining the pathogen, cell structures, and starch grains. The number of starch (amylopectin) grains increased in B. rapa roots infected by P. brassicae, especially from 14 to 21 days after inoculation. Therefore, the expression levels of 38 core starch metabolism genes were investigated by quantitative real-time PCR. Most genes related to starch synthesis were up-regulated at 7 days after P. brassicae inoculation, whereas the expression levels of starch degradation-related genes were increased at 14 days after inoculation. Then, genes encoding the core enzymes involved in starch metabolism were investigated by assessing their chromosomal distributions, structures, duplication events, and synteny among Brassica species. Genome comparisons indicated that 38 non-redundant genes belonging to six core gene families related to starch metabolism are highly conserved among Arabidopsis thaliana, B. rapa, Brassica nigra, and Brassica oleracea. Previous genome sequencing projects have revealed that P. brassicae obtained host nutrients by manipulating plant metabolism. Starch may serve as a carbon source for P. brassicae colonization, as indicated by histological observations and transcriptomic analysis. Results of this study may elucidate the evolution and expression of core starch metabolism genes and provide researchers with novel insights into the pathogenesis of clubroot in B. rapa.