文献类型： 外文期刊

作者： Liu, Jiawei ¹ ; Hao, Xinyan ¹ ; Guo, Yue ³ ; Wang, Mingjiu ⁴ ; Huang, Fan ² ;

作者机构： 1.Inner Mongolia Acad Agr & Anim Husb Sci, Inst Plant Protect, Hohhot, Peoples R China

2.Chinese Acad Agr Sci, Inst Grassland Res, Hohhot, Peoples R China

3.Inner Mongolia Univ Finance & Econ, Res Dept, Hohhot, Peoples R China

4.Inner Mongolia Agr Univ, Coll Grassland Sci, Hohhot, Peoples R China

关键词： Caucasian clover; nodulation; nitrogen fixation; transcriptomics; proteomics

期刊名称：FRONTIERS IN GENETICS （ 影响因子：2.8； 五年影响因子：3.3 ）

ISSN：

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： Introduction Caucasian clover (Trifolium ambiguum Bieb.), a perennial legume forage grass, exhibits strong adaptability and resistance to adverse conditions. Owing to its rhizome specificity, it cannot nodulate or fix nitrogen outside of its original location, which limits its promotion and cultivation. The phenomenon of spontaneous nodulation of Caucasian clover was observed for the first time in Inner Mongolia, and investigation of its nodulation and nitrogen fixation mechanisms assists in the subsequent promotion of Caucasian clover cultivation from a theoretical perspective.Methods In this study, rhizobia extracted from Caucasian clover were inoculated into the field, and the nodulation part of the root system was investigated during the regreening stage of the second year using transcriptomics and proteomics techniques.Results and Discussion The study identified 70,280 differentially expressed genes (DEGs) and 770 differentially expressed proteins (DEPs) in total, among which carbonic anhydrase, cyanate lyase, phenylalanine, caffeate/5-hydroxyferulate 3-O-methyltransferase, caffeoyl CoA 3-O methyltransferase, chalcone synthase, and chalcone isomerase may have been the main factors affecting the nodulation and nitrogen fixation of Caucasian clover. This study theoretically contributes to the future genetic validation and selection of Caucasian clover varieties with a strong ability to nodulate and fix nitrogen.