您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterization and genome analysis of a broad lytic spectrum bacteriophage P479 against multidrug-resistant Escherichia coli

文献类型： 外文期刊

作者： Li, Pei 1 ; Wang, Hui 1 ; Li, Min 1 ; Qi, Weiling 1 ; Qi, Zitai 1 ; Chen, Weiye 1 ; Dong, Yongyi 5 ; Xu, Zhengjun 5 ; Zhang, Wei 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, 1 Weigang, Nanjing 210095, Peoples R China

2.Minist Agr, Key Lab Anim Bacteriol, 1 Weigang, Nanjing 210095, Peoples R China

3.OIE Reference Lab Swine Streptococcosis, 1 Weigang, Nanjing 210095, Peoples R China

4.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, 1 Weigang, Nanjing 210095, Peoples R China

5.Jiangsu Anim Dis Control Ctr, 124 Caochangmen St, Nanjing 210036, Peoples R China

关键词： Bacteriophage; E. coli; Multiple drug resistance; Broad lytic spectrum; Genome sequencing; Serotypes

期刊名称：VIRUS RESEARCH （ 影响因子：6.286； 五年影响因子：4.486 ）

ISSN： 0168-1702

年卷期： 2022 年 308 卷

页码：

收录情况： SCI

摘要： The increase of multi-drug resistant and multi-serotypes of pathogenic Escherichia coli has brought more severe challenge to control infection. Nowadays, bacteriophage is a promising tool to treat colibacillosis as an alternative of antibiotics. A coliphage P479, isolated from sewage of poultry farm, could lyse multiple serotypes, including not only O1, O2, O8, O9, O21, O78, O83, O145 of Avian pathogenic E. coli, but O157:H7 of Enterohaemorrhagic E. coli and O18:K1:H7 Neonatal meningitis E. coli. Additionally, P479 could also lyse multi-drug resistant E. coli. These indicated that P479 had good lytic ability. One-step growth curve revealed that the latent time period of P479 was 10 min and the burst size was about 318 PFU/cell. Stability tests demonstrated that P479 had good stability under various temperature (4 to 50 degrees C) and pH (3 to11) conditions. P479 contained of a linear, double-stranded DNA molecule of 172,033 bp with 40.3% GC content. P479 contained 296 putative coding sequences (CDSs) and two tRNA genes. Based on genomic comparison, P479 was classified as a member of genus Gaprivervirus, subfamily Tevenvirinae, family Myoviridae, order Caudovirales. No known virulent or lysogenic genes were detected in the genome of P479, manifesting P479 was safe to adhibit. Antibacterial activity in vitro manifested that P479 has varying degrees bacteriostatic activity against different bacteria. According to the above properties, P479 has the potential to be applied in phage therapy in the future.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台