Exploring Disparities in Gill Physiological Responses to NaHCO3-Induced Habitat Stress in Triploid and Diploid Crucian Carp (Carassius auratus): A Comprehensive Investigation Through Multi-Omics and Biochemical Analyses
文献类型： 外文期刊
作者： Han, Shicheng 1 ; Han, Lin 1 ; Yuan, Fangying 1 ; Liu, Wenzhi 1 ; Wang, Jing 3 ; Jin, Xiaofeng 1 ; Sun, Yanchun 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Minist Agr & Rural Areas, Heilongjiang River Fisheries Res Inst, Lab Qual & Safety Risk Assessment Aquat Prod Harbi, Harbin 150070, Peoples R China
2.Shanghai Ocean Univ, Coll Food Sci & Technol, Dept Food Sci & Engn, Shanghai 201306, Peoples R China
3.Harbin Univ Sci & Technol, Coll Mat & Chem Engn, Dept Chem Engn & Technol, Harbin 150080, Peoples R China
关键词：
crucian carp (
期刊名称：METABOLITES （ 影响因子：3.7； 五年影响因子：4.1 ）
ISSN：
年卷期： 2025 年 15 卷 1 期
页码：
收录情况： SCI
摘要： Background: Owing to the progressive rise in saline waters globally, resulting in detrimental impacts on freshwater aquaculture, the underlying molecular distinctions governing the response to alkaline stress between diploid and triploid crucian carp remain unknown. Methods: This investigation explores the effects of 20 and 60 mmol NaHCO3 stress over 30 days on the gills of diploid and triploid crucian carp, employing histological, biochemical, and multi-omic analyses. Results: Findings reveal structural damage to gill lamellas in the examined tissue. Diploid crucian carp exhibit heightened activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and alkaline phosphatase (AKP), alongside lower malondialdehyde (MDA) and urea nitrogen (BUN) levels compared to triploid counterparts. Metabolomic investigations suggest alterations in purine metabolism, lipid metabolism, sphingolipid metabolism, and aminoglycan and nucleotide sugar metabolism following NaHCO3 exposure. Transcriptomic data indicate differential expression of genes associated with nitrogen metabolism, complement and coagulation cascades, IL-17 signaling pathways, and Toll-like receptor signaling pathways. Conclusions: Overall, NaHCO3-induced stress leads to significant gill tissue damage, accompanied by reactive oxygen species (ROS) production causing oxidative stress and disruptions in lipid metabolism in crucian carp. Furthermore, an inflammatory response in gill cells triggers an immune response. Diploid crucian carp exhibit superior antioxidant and immune capacities compared to triploid counterparts, while also displaying reduced inflammatory responses in vivo. Notably, diploid carp efficiently excrete excess BUN through purine metabolism, mitigating protein metabolism and amino acid imbalances caused by BUN accumulation. This enables them to allocate less energy for coping with external environmental stress, redirecting surplus energy toward growth and development. The above results indicate that diploid organisms can better adapt to saline-alkaline environments. Overall, this study provides novel perspectives into species selection of crucian carp of different ploidy in saline-alkaline waters.
- 相关文献
作者其他论文 更多>>
-
Dual Regulation of Ionic Effect on Zostera marina L. Seed Germination and Leaf Differentiation in Low-Salinity Conditions
作者：Li, Peilong;Gao, Yaping;Jiang, Zengjie;Wang, Linjie;Li, Peilong;Jiang, Zengjie;Sun, Xiaoli;Wang, Jiaqi;Wang, Jing;Sui, Haidong;Wang, Junwei;Zhang, Yitao
关键词：germination; leaf differentiation; osmotic pressure; biochemical traits; antioxidant enzymes
-
Antiviral activity of arctigenin against Chinese rice-field eel rhabdovirus in Monopterus albus
作者：Liu, Yisha;Xue, Mingyang;Xu, Chen;Zhou, Yong;Jiang, Nan;Meng, Yan;Li, Yiqun;Huang, Zhenyu;Liu, Wenzhi;Li, Zhong;Fan, Yuding;Liu, Yisha;Zhong, Qiwang
关键词：Arctigenin; Rhabdovirus; Monopterus albus; Antiviral
-
Viperin and Its Effect on SVCV Replication in Common Carp, Cyprinus carpio
作者：Meng, Yan;Hu, Xi;Jiang, Nan;Fan, Yuding;Li, Yiqun;Xue, Mingyang;Xu, Chen;Liu, Wenzhi;Zhou, Yong
关键词：common carp (
Cyprinus carpio );viperin ; characteristics; antiviral activities -
Pharmacokinetics, Withdrawal Time, and Physiological Effects of Single Oral Administration of Enrofloxacin in Dybowski's Frog (Rana dybowskii)
作者：Wang, Yanan;Wang, Jing;Zhao, Ran;Li, Shaowu;Hu, Guo;Wang, Di;Wang, Yanan
关键词：
Rana dybowskii ; enrofloxacin; ciprofloxacin; pharmacokinetic; withdrawal time -
Comprehensive Evaluation of Growth Performance, Hematological Parameters, Antioxidant Capacity, Innate Immunity, and Disease Resistance in Crucian Carp (Carassius auratus) Lacking Intermuscular Bones
作者：Zhao, Ran;Zhu, Jianqiang;Li, Shaowu;Sun, Zhipeng;Zhang, Tingting;Wang, Jing;Zheng, Xianhu;Kuang, Youyi;Wang, Di
关键词：Carassius auratus; a new strain lacking intermuscular bones; growth performance; iron overload; immune function
-
Multi-omics-based study on metabolic-physiological response to heat stress in rainbow trout (Oncorhynchus mykiss)
作者：Yang, Yang;Wu, Song;Sun, Yanchun;Han, Shicheng;Liu, Yingjie;Liu, Wenzhi;Han, Lin;Yuan, Fangying;Wang, Jing
关键词：Rainbow trout; Heat stress; Multi-omics; Adaptive strategies; Inflammation response
-
Immunological Responses and Protection in the Largemouth Bass (Microterus salmoides) Immunized with Inactivated Vaccine Against Largemouth Bass Ranavirus (LMBRaV)
作者：Yang, Tao;Zhai, Jiale;Zeng, Lingbing;Zhou, Yong;Yang, Tao;Li, Chenyang;Yang, Tao;Zhai, Jiale;Zeng, Lingbing;Li, Yiqun;Liu, Wenzhi;Meng, Yan;Fan, Yuding;Huang, Zhenyu;Zhou, Yong;Jiang, Nan
关键词：largemouth bass; largemouth bass ranavirus; inactivated vaccine; relative percent survival rates; immune response
