Nile tilapia TLR3 recruits MyD88 and TRIF as adaptors and is involved in the NF-?B pathway in the immune response
文献类型： 外文期刊
作者： Gao, Fengying 1 ; Pang, Jicai 1 ; Lu, Maixin 1 ; Liu, Zhigang 1 ; Wang, Miao 1 ; Ke, Xiaoli 1 ; Yi, Mengmeng 1 ; Cao, Jianmeng 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat &, Minist Agr, Guangzhou 510380, Peoples R China
2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangdong Prov Key Lab Aquat Anim Immune Technol, Guangzhou 510380, Peoples R China
3.Shandong Vocat Anim Sci & Vet Coll, Weifang 261021, Shandong, Peoples R China
4.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, 1 Xing Yu Rd, Guangzhou 510380, Guangdong, Peoples R China
关键词： Nile tilapia ( Oreochromis niloticus ); TLR3; MyD88 and TRIF adaptor
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）
ISSN： 0141-8130
年卷期： 2022 年 218 卷
页码：
收录情况： SCI
摘要： TLR3 plays a crucial role in innate immunity. In the present study, OnTLR3 was identified in the Nile tilapia Oreochromis niloticus, with a conserved LRR domain and a C-terminal TIR domain. OnTLR3 was broadly expressed in all tissues tested, with the highest expression levels in the blood and the lowest in the kidney. TLR3 mRNA could be detected from pharyngula (2.5 dpf) to late larva (8.5 dpf) during embryonic and larval development. Moreover, the expression level of OnTLR3 was clearly altered in all five tissues after Streptococcus agalactiae infection in vivo and could be induced by LPS, poly(I:C), S. agalactiae WC1535 and delta CPS in Nile tilapia macrophages. When OnTLR3 was overexpressed in 293 T cells, it was distributed in the cytoplasm and could significantly increase NF-kappa B activation. The pulldown assays showed that OnTLR3 interacted with both OnMyD88 and OnTRIF. The binding assays revealed the specificity of OnTLR3 for pathogen-associated molecular patterns (PAMPs) and bacteria that included S. agalactiae, Aeromonas hydrophila and poly(I:C), LPS and PGN. Taken together, these findings suggest that OnTLR3, as a pattern recognition receptor (PRR), might play an important role in the immune response to pathogen invasion.
- 相关文献
作者其他论文 更多>>
-
A multiple-population study reveals genetic variation and genes associated with resistance to Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus)
作者：Wang, Zhang;Liu, Zhigang;Huang, Shaowei;Zhu, Weijuan;Cao, Jianmeng;Wang, Miao;Yi, Mengmeng;Lu, Maixin;Ke, Xiaoli;Liu, Zhigang;Huang, Shaowei;Zhu, Weijuan;Lu, Maixin;Ke, Xiaoli
关键词：Tilapia; Streptococcus agalactiae; GWAS; Selective sweep analysis; SNP marker
-
Chromosome-level genome assembly of Jaguar guapote (Parachromis manguensis) by massive parallel sequencing
作者：Cao, Jianmeng;Chen, Huizi;Liu, Zhigang;Tong, Yannan;Xiao, Zhigang
关键词：
-
Integrated gut microbiome and serum metabolomics analysis to reveal seawater adaptation strategy in Chinese sturgeon
作者：Leng, Xiaoqian;Luo, Jiang;Zhong, Jia;Wu, Jinping;Qiao, Xinmei;Xiong, Wei;Liu, Zhigang;Du, Hao
关键词：Seawater acclimation; 16S rRNA sequencing; Correlation analysis; Carbohydrate metabolism
-
Comparative analysis of the intestinal flora of Siniperca chuatsi at different growth stages under three feeding practices
作者：Yan, Yunyun;Chen, Xiao;Dong, Junjian;Gao, Fengying;Zhang, Hetong;Ye, Xing;Sun, Chengfei;Yan, Yunyun;Wu, Chengbin;Wang, Fubao
关键词：Compound feed; Live bait; Intestinal flora; 16S rRNA sequencing; Siniperca chuatsi
-
Nile tilapia P38 MAPK interacts with Tak1 and is involved in the immune response to Streptococcus agalactiae, PolyI:C, and LPS stimulation
作者：Zheng, Qiuyue;Liu, Zhigang;Sun, Chengfei;Dong, Junjian;Zhang, Hetong;Ke, Xiaoli;Gao, Fengying;Lu, Maixin;Zheng, Qiuyue
关键词：Nile tilapia ( Oreochromis niloticus ); P38 MAPK; Immune response; Expression profile
-
Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Mechanisms Involved in the Adaptations of Mandarin Fish (Siniperca chuatsi) to Compound Feed
作者：Yan, Yunyun;Zhang, Yuan;Dong, Junjian;Zhang, Hetong;Gao, Fengying;Ye, Xing;Sun, Chengfei;Yan, Yunyun;Wu, Chengbin;Wang, Fubao
关键词：
Siniperca chuatsi ; compound feed; transcriptome; metabolome; integrated analysis -
Clock gene NR1D1 mediated inflammatory response after Streptococcus agalactiae challenge in Nile Tilapia (Oreochromis niloticus) brain
作者：Wang, Zhang;Liu, Zhigang;Zhu, Weijuan;Zhang, Meiyan;Huang, Shaowei;Cao, Jianmeng;Wang, Miao;Yi, Mengmeng;Gao, Fengying;Lu, Maixin;Ke, Xiaoli;Liu, Zhigang;Zhu, Weijuan;Zhang, Meiyan;Huang, Shaowei;Gao, Fengying;Lu, Maixin;Ke, Xiaoli
关键词：S. agalactiae; Neuronal injury; NF-kappa B; Astrocytes inflammatory response
