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Transcription factors AcERF74/75 respond to waterlogging stress and trigger alcoholic fermentation-related genes in kiwifruit

文献类型： 外文期刊

作者： Liu, Jiao 1 ; Chen, Yue 1 ; Wang, Wen-qiu 1 ; Liu, Ji-hong 3 ; Zhu, Chang-qing 1 ; Zhong, Yun-peng 4 ; Zhang, Hui-qin 5 ; Liu, Xiao-fen 1 ; Yin, Xue-ren 1 ;

作者机构： 1.Zhejiang Univ, Coll Agr & Biotechnol, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Zijingang Campus, Hangzhou 310058, Peoples R China

2.Zhejiang Univ, State Agr Minist, Lab Hort Plant Growth Dev & Qual Improvement, Zijingang Campus, Hangzhou 310058, Peoples R China

3.Huazhong Agr Univ, Coll Hort & Forestry Sci, Key Lab Hort Plant Biol, Wuhan 430070, Peoples R China

4.Chinese Acad Agr Sci, Zhengzhou Fruit Res Inst, Key Lab Fruit Tree Growth Dev & Qual Control, Zhengzhou 450009, Peoples R China

5.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Peoples R China

关键词： Kiwifruit; Waterlogging stress; RNA-seq; ERF-VIIs; ADH

期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）

ISSN： 0168-9452

年卷期： 2022 年 314 卷

页码：

收录情况： SCI

摘要： Kiwifruit plants have a fleshy, shallow root system which is sensitive to waterlogging stress, which results in a decrease in crop yield or even plants death. Although the waterlogging stress responses in kiwifruit have attracted much attention, the underlying molecular mechanism remains unclear. In this study, waterlogging led to drastic inhibition of root growth of 'Donghong' kiwifruit (Actinidia chinensis) plants grown in vitro, which was accompanied by significant elevation of endogenous acetaldehyde and ethanol contents. RNA-seq of roots of plants waterlogged for 0, 1 and 2 days revealed that a total of 149 genes were up- or down-regulated, including seven biosynthetic genes related to the glycolysis/gluconeogenesis pathway and 10 transcription factors. Analyses with real-time PCR, dual-luciferase assays and EMSA demonstrated that AcERF74 and AcERF75, two members of the ERF-VII subfamily, directly upregulated AcADH1 (alcohol dehydrogenase). Moreover, the overexpression of AcERF74/75 in transgenic calli resulted in dramatic increase of endogenous ethanol contents through the triggering of AcADH1 and AcADH2 expression. Although the AcPDC2 (pyruvate decarboxylase) expression was also enhanced in transgenic lines, the endogenous acetaldehyde contents showed no significant changes. These results illustrated that AcERF74/75 are two transcriptional activators on alcoholic fermentation related genes and are responsive to waterlogging stress in kiwifruit.

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[1]Transcription factors AcERF74/75 respond to waterlogging stress and trigger alcoholic fermentation-related genes in kiwifruit. Liu, Jiao,Chen, Yue,Wang, Wen-qiu,Zhu, Chang-qing,Liu, Xiao-fen,Yin, Xue-ren,Liu, Jiao,Chen, Yue,Wang, Wen-qiu,Zhu, Chang-qing,Liu, Xiao-fen,Yin, Xue-ren,Liu, Ji-hong,Zhong, Yun-peng,Zhang, Hui-qin. 2022

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