Sterol 14 alpha-Demethylase CaCYP51A and CaCYP51B are Functionally Redundant, but Differentially Regulated in Colletotrichum acutatum: Responsibility for DMI-Fungicide Resistance
文献类型： 外文期刊
作者： Wei, Lingling 1 ; Li, Xiujuan 1 ; Chen, Bin 1 ; Chen, Wenchan 2 ; Wei, Lihui 2 ; Zhou, Dongmei 2 ; Chen, Changjun 1 ; Wu, Chengdong 3 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210095, Jiangsu, Peoples R China
3.Nanjing Pukou Dist Agr Technol Extens Ctr, Nanjing 211800, Jiangsu, Peoples R China
关键词： anthracnose; Colletotrichum acutatum; tebuconazole; resistance mechanism; CaCYP51 genes
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.895； 五年影响因子：6.048 ）
ISSN： 0021-8561
年卷期：
页码：
收录情况： SCI
摘要： Colletotrichum acutatum, the main pathogen causing anthracnose on chili worldwide, is controlled by tebuconazole [a sterol C14-demethylation inhibitor (DMI) fungicide, abbreviated as Teb] with excellent efficacy. Our previous study exhibited that all C. acutatum isolates were sensitive to Teb while the Colletotrichum gloeosporioides population had developed resistance to Teb on the same fungicide-pressure selection. Therefore, the assessment of Teb-resistance in C. acutatum is impending. Twenty Tebresistant (Teb) mutants obtained by fungicide domestication and ultraviolet (UV)-mutagenesis displayed similar fitness compared to parental isolates. Data in the current study exhibited that mutations at CaCYP51A and/or overexpression of CaCYP51s were responsible for Teb-resistance. Furthermore, the deletion mutants Delta CaCYP51A and Delta CaCYP51B played different roles in sensitivities to DMIs. Taken together, this study first reported that mutations at CaCYP51A and/or overexpression of CaCYP51s conferred resistance to Teb in C. acutatum, CaCYP51A and CaCYP51B are functionally redundant, but differentially regulated in DMI resistance.
- 相关文献
作者其他论文 更多>>
-
The natural polycyclic tetramate macrolactam HSAF inhibit Fusarium graminearum through altering cell membrane integrity by targeting FgORP1
作者：Chen, Wenchan;Tang, Bao;Hou, Rongxian;Sun, Weibo;Guo, Baodian;Zhao, Yangyang;Li, Chaohui;Sheng, Cong;Zhao, Yancun;Liu, Fengquan;Liu, Fengquan;Han, Chenyang
关键词：F. graminearum; HSAF; FgORP1; Ergosterol; Cell membrane
-
Staurosporine-producing Streptomyces sp. strain 11 x 1 cell-free culture filtrates control diseases caused by the oomycete plant pathogens Pythium myriotylum and Phytophthora sojae
作者：Wen, Xian;Wang, Lunji;Li, Jingjing;Jiu, Min;Wen, Xian;Li, Jingjing;Chen, Yifan;Zhuo, Deyu;Anjago, Wilfred Mabeche;Zhu, Hongli;Zhang, Qimeng;Zhang, Jinfeng;Wang, Xiaoyu;Wei, Lihui;Daly, Paul;Zhou, Dongmei;Chen, Yifan;Wei, Lihui;Peng, Hao;Wang, Lunji;Daly, Paul;Zhou, Dongmei
关键词：Streptomyces; Pythium myriotylum; Phytophthora sojae; Biological control; Ginger; Soybean
-
The HOG-pathway related AaOS1 leads to dicarboximide-resistance in field strains of Alternaria alternata and contributes, together with the Aafhk1, to mycotoxin production and virulence
作者：Chen, Changjun;Wang, Xiaoyu;Wei, Lingling;Chen, Bin;Li, Xiujuan;Shi, Haiping;Xie, Shuai;Hu, Hao;Chen, Changjun;Chen, Wenchan;Wei, Lihui;Wang, Xiaoyu
关键词：garlic leaf spot; Alternaria alternata; dicarboximides; mutations; mycotoxin AOH
-
Functional Plasticity, Redundancy, and Specificity of Lanosterol 14α-Demethylase in Regulating the Sensitivity to DMIs in Calonectria ilicicola
作者：Wei, Lingling;Shi, Haiping;Chen, Bin;Li, Xiujuan;Chen, Changjun;Chen, Wenchan;Wu, Chengdong;Gai, Yunpeng
关键词：C. ilicicola; sterol 14 alpha-demethylase; novel genus-specific CYP51 function; DMI sensitivity; molecular docking
-
A major gene for chilling tolerance variation in Indica rice codes for a kinase OsCTK1 that phosphorylates multiple substrates under cold
作者：Wu, Jiawen;Liu, Huimin;Zhang, Yan;Zhang, Yingdong;Li, Dongling;Liu, Shiyan;Lu, Shan;Zou, Baohong;Liu, Huimin;Zhang, Yan;Wei, Lihui;Hua, Jian
关键词：chilling tolerance; GWAS; haplotype; natural variation; OsCTK1; phosphorylation
-
Resistance mechanism of Phomopsis longicolla to fludioxonil is associated with modifications in PlOS1, PlOS4 and PlOS5
作者：Wei, Lingling;Chen, Bin;Li, Jiawei;Zhang, Pengcheng;Ye, Wenwu;Chen, Changjun;Chen, Wenchan
关键词：P. longicolla; Fludioxonil; Mutation in PlOSs; Hog1 phosphorylation level; Baseline sensitivity; Resistance risk
-
Growth, Enzymatic, and Transcriptomic Analysis of xyr1 Deletion Reveals a Major Regulator of Plant Biomass-Degrading Enzymes in Trichoderma harzianum
作者：Wang, Lunji;Zhao, Yishen;Wen, Xian;Jiu, Min;Zhao, Yishen;Chen, Siqiao;Wen, Xian;Anjago, Wilfred Mabeche;Tian, Tianchi;Chen, Yajuan;Zhang, Jinfeng;Deng, Sheng;Zhou, Dongmei;Wei, Lihui;Daly, Paul;Chen, Siqiao;Chen, Yajuan;Fu, Pengxiao;Druzhinina, Irina S.
关键词：CAZymes; XYR1/XlnR/XLR-1; cellulose; transcriptional regulation
