Transcriptome sequence reveal the roles of MaGME777 and MabHLH770 in tolerance in Musa acuminata
文献类型： 外文期刊
作者： Wei, Yuchen 1 ; Shi, Jingfang 6 ; Xie, Xueyi 7 ; Zhang, Feng 1 ; Dong, Huizhen 1 ; Li, Yaoyao 2 ; Bi, Fangcheng 2 ; Huang, Xiaosan 1 ; Dou, Tongxin 2 ;
作者机构： 1.Nanjing Agr Univ, Sanya Inst, Sanya 572025, Hainan, Peoples R China
2.Guangdong Prov Key Lab Sci & Technol Res Fruit Tre, Guangzhou 510640, Guangdong, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangzhou 510640, Peoples R China
4.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Guangdong, Peoples R China
5.Nanjing Agr Univ, Ctr Pear Engn Technol Res, State Key Lab Crop Genet & Gemplasm Enhancement, Nanjing 210095, Peoples R China
6.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Prov Key Lab Crop Germplasm Resources Pr, Guangzhou 510640, Guangdong, Peoples R China
7.Guangzhou Univ, Sch Life Sci, Guangzhou 510006, Guangdong, Peoples R China
关键词： Cavendish banana; Drought stress; RNA-Seq; WGCNA; BHLH
期刊名称：PLANT SCIENCE （ 影响因子：4.1； 五年影响因子：5.1 ）
ISSN： 0168-9452
年卷期： 2025 年 356 卷
页码：
收录情况： SCI
摘要： Banana, a globally cultivated fruit, faces significant constraints in distribution and sustainable production due to drought stress. This study investigated drought tolerance in Cavendish bananas using RNA-seq time-course analysis and molecular biology experiments. Plants were subjected to dehydration treatments, and physiological indicators such as electrolyte leakage, proline content, malonaldehyde, peroxidase activity, and hydrogen peroxide content were assessed. RNA-Seq and qRT-PCR were used to analyze transcriptional changes under drought. Weighted gene co-expression network (WGCNA) analysis identified thousands of differentially expressed genes (DEGs) at different time points, with a core set of 2660 DEGs consistently identified. KEGG enrichment analysis revealed MaGME777, a glycolysis/gluconeogenesis gene, as a potential drought resistance regulator. Virus-mediated gene silencing (VIGS) of MaGME777 reduced drought tolerance in bananas. Yeast onehybrid (Y1H) and luciferase reporter assays demonstrated that the transcription factor MabHLH770 directly binds and activates the MaGME777 promoter. VIGS downregulation of MabHLH770 also reduced drought tolerance. In conclusion, this study revealed that MabHLH770 is a positive regulator of drought stress, by targeting MaGME777 promoter and activating their expression to enhance drought tolerance. These findings provide a foundation for developing drought-resistant banana cultivars through molecular breeding approaches.
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