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Screening, identification, and mechanistic exploration of DPP-IV inhibitory peptides from collagen in Esox lucius skin

文献类型： 外文期刊

作者： Li, Jialu 1 ; Wei, Yabo 1 ; Lei, Yongdong 4 ; Guo, Xin 1 ; Zhao, Yindi 1 ; Hao, Qi 1 ; Deng, Xiaorong 1 ; Zhang, Jian 1 ;

作者机构： 1.Shihezi Univ, Sch Food Sci & Technol, Shihezi 832003, Xinjiang, Peoples R China

2.Shihezi Univ, Sch Food Sci & Technol, Key Lab Agr Prod Proc & Qual Control Specialty, Coconstruct Minist & Prov, Shihezi 832003, Xinjiang, Peoples R China

3.Shihezi Univ, Sch Food Sci & Technol, Key Lab Food Nutr & Safety Control Xinjiang Prod &, Shihezi 832003, Xinjiang, Peoples R China

4.Xinjiang Acad Agr & Reclamat Sci, Minist Agr & Rural Affairs, Food Qual & Testing Ctr, Shihezi 832003, Xinjiang, Peoples R China

关键词： Esox lucius; DPP-IV inhibitory peptide; Molecular docking; Virtual screening

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.9； 五年影响因子：6.1 ）

ISSN： 2212-4292

年卷期： 2025 年 63 卷

页码：

收录情况： SCI

摘要： Food-derived dipeptidyl peptidase IV (DPP-IV) inhibitors show promise as a therapeutic intervention for type 2 diabetic mellitus (T2DM). This investigation used a combination of virtual screening and enzymatic digestion to prepare and identify novel DPP-IV inhibitory peptides from Esox lucius, a typical cold-water fish from Xinjiang. Alkaline protease digest showed the best inhibitory effect of 62.04% +/- 1.07%. The ultrafiltration U1 (<3 kDa) fraction had the best inhibitory activity with an IC50 value of 2.77 +/- 0.09 mg/mL. From the dextran gel chromatography S2 fraction, 543 peptide fragments were found, and six peptides underwent virtual screening. Among them, APDPF showed promising DPP-IV inhibitory activity with an IC50 value of 0.38 +/- 0.02 mg/mL and exhibited competitive inhibition of DPP-IV by binding to amino acid residues in the S1 and S2 active pockets by forming hydrogen bonds and hydrophobic forces. This study provides a new way to prepare DPP-IV inhibitory peptide and is of great theoretical significance and practical value for further exploring the development of endogenous peptides from Esox lucius.

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