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Physiological, transcriptomic, and metabolomic analyses reveal that Pantoea sp. YSD J2 inoculation improves the accumulation of flavonoids in Cyperus esculentus L. var. sativus

文献类型： 外文期刊

作者： Wang, Saisai 1 ; Huang, Yanna 1 ; Sun, Yu 4 ; Wang, Jinbin 4 ; Tang, Xueming 1 ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Agr & Biol, Dongchuan Rd 800, Shanghai 200240, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Urban Agr, Dongchuan Rd 800, Shanghai 200240, Peoples R China

3.Minist Sci & Technol, Minist Educ, Shanghai Yangtze River Delta Ecoenvironm Change &, Dongchuan Rd 800, Shanghai 200240, Peoples R China

4.Shanghai Acad Agr Sci, Key Lab Agr Genet & Breeding, Biotechnol Res Inst, Beidi Rd 2901, Shanghai 201106, Peoples R China

关键词： Tigernut; Plant growth-promoting microorganisms; Flavonoid biosynthesis; Metabolome; Transcriptome

期刊名称：HELIYON （ 影响因子：3.4； 五年影响因子：3.9 ）

ISSN：

年卷期： 2024 年 10 卷 16 期

页码：

收录情况： SCI

摘要： Plant growth-promoting microorganisms (PGPMs), such as Pantoea sp. YSD J2, promote plant development and stress resistance, while their role in flavonoids accumulation still needs to be further understood. To investigate the complex flavonoid biosynthesis pathway of Cyperus esculentus L. var. sativus (tigernut), we compared Pantoea sp. YSD J2 inoculation (YSD J2) and water inoculation (CK) groups. YSD J2 significantly elevated the content of indole-3-acetic acid (IAA) and orientin. Furthermore, when analyzing flavonoid metabolome, YSD J2 caused increased levels of uralenol, petunidin-3-O-glucoside-5-O-arabinoside, O-glucoside-5- O-arabinoside, luteolin-7-O-glucuronide-(2 O-glucuronide-(2 -> 1)glucuronide, kaempferol-3-O-neohesperidoside, O-neohesperidoside, cyanidin-3-O-(2 ''-O-glucosyl)glucoside, O-(2 ''- O-glucosyl)glucoside, kaempferol-3-O-glucuronide-7-O-glucoside, O-glucuronide-7- O-glucoside, quercetin-3-O-glucoside, O-glucoside, luteolin-7-O-glucuronide- O-glucuronide- (2 -> 1)-(2 ''-sinapoyl)glucuronide, ''-sinapoyl)glucuronide, and quercetin-4 '-O-glucoside, '- O-glucoside, which further enhanced antioxidant activity. We then performed RNA-seq and LC-MS/MS, aiming to validate key genes and related flavonoid metabolites under YSD J2 inoculation, and rebuild the gene-metabolites regulatory subnetworks. Furthermore, the expression patterns of the trans cinnamate 4-monooxygenase ( CYP73A ), flavonol-3-O-L-rhamnoside-7-O-glucosyltransferase O-L-rhamnoside-7- O-glucosyltransferase ( UGT73C6 ), shikimate O- hydroxycinnamoyltransferase (HCT), HCT ), chalcone isomerase (CHI), CHI ), flavonol synthase (FLS), FLS ), and anthocyanidin synthase (ANS) ANS ) genes were confirmed by qRT-PCR. Additionally, 4 transcription factors (TF) (especially bHLH34, Cluster-37505.3) under YSD J2 inoculation are also engaged in regulating flavonoid accumulation. Moreover, the current work sheds new light on studying the regulatory effect of Pantoea sp. YSD J2 on tigernut development and flavonoid biosynthesis.

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