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Clearance of bacteria from lymph nodes in sheep immunized with Brucella suis S2 vaccine is associated with M1 macrophage activation

文献类型： 外文期刊

作者： Chen, Si 1 ; Chen, Yuanyuan 1 ; Jiao, Zizhuo 1 ; Wang, Chengqiang 1 ; Zhao, Dantong 2 ; Liu, Yongbin 3 ; Zhang, Wenguang 4 ; Zhao, Shihua 5 ; Yang, Bin 5 ; Zhao, Qinan 5 ; Fu, Shaoyin 5 ; He, Xiaolong 5 ; Chen, Qiaoling 1 ; Man, Churiga 1 ; Liu, Guoying 2 ; Wei, Xuefeng 2 ; Du, Li 1 ; Wang, Fengyang 1 ;

作者机构： 1.Hainan Univ, Sch Anim Sci & Technol, Hainan Key Lab Trop Anim Reprod, Anim Genet Engn Key Lab Haikou, Haikou, Hainan, Peoples R China

2.Jinyu Baoling Biopharmaceut Co Ltd, Hohhot, Peoples R China

3.Inner Mongolia Univ, Coll Rd 235, Hohhot, Peoples R China

4.Inner Mongolia Agr Univ, Coll Life Sci, Hohhot, Peoples R China

5.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot, Peoples R China

关键词： Brucella; sheep; vaccine; lymphatic nodes; macrophages

期刊名称：VETERINARY RESEARCH （ 影响因子：4.4； 五年影响因子：4.3 ）

ISSN： 0928-4249

年卷期： 2023 年 54 卷 1 期

页码：

收录情况： SCI

摘要： Ovine brucellosis is a global zoonotic disease of sheep caused by Brucella melitensis, which inflicts a significant burden on human and animal health. Brucella suis strain S2 (B. suis S2) is a smooth live attenuated vaccine for the prevention of ovine brucellosis in China. However, no previous studies have assessed the immunogenicity of B. suis S2 vaccine after oral immunization in sheep. Here, we attempted to evaluate the ovine immune response over the course of B. suis S2 immunization and to identify in vivo predictors for vaccine development. Body temperature, serum Brucella antibodies, serum cytokines (IL-12p70 and interferon [IFN]-gamma), and bacterial load in the mandibular lymph nodes (LN), superficial cervical LN, superficial inguinal LN, and spleen were investigated to determine the safety and efficacy of the vaccine. The abnormal body temperature of sheep occurred within 8 days post-infection (dpi). Brucella suis S2 persisted for a short time (< 21 dpi) in the mandibular LN. The highest level of IL-12p70 was observed at 9 dpi, whereas serum IFN-gamma levels peaked at 12 dpi. Transcriptome analysis and quantitative reverse transcription PCR were performed to determine gene expression profiles in the mandibular LN of sheep. Antigen processing and presentation pathway was the dominant pathway related to the dataset. Our studies suggest that the immune response in ovine LN resembled type 1 immunity with the secretion of IL-12p70 and IFN-gamma after B.suis S2 immunization and the vaccine may eliminate Brucella via stimulation of M1 macrophages through the course of Th cells.

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