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Transcriptome analysis reveals the feeding response and oxidative stress in juvenile Micropterus salmoides fed a low-fish-meal diet with enzyme-hydrolysed intestinal mucosa protein substitution

文献类型： 外文期刊

作者： Yu, Heng 1 ; Ge, Xianping 1 ; Zhang, Lin 2 ; Chen, Xiaoru 3 ; Ren, Mingchun 1 ; Liang, Hualiang 1 ;

作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China

2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Integrated Rice Fish Farming Ecol, Minist Agr & Rural Affairs, Wuxi 214081, Peoples R China

3.Tongwei Agr Dev Co LTD, Chengdu 610041, Peoples R China

关键词： M; salmoides; Enzyme-hydrolysed intestinal mucosa protein; Transcriptome analysis; Feeding response; Oxidative stress

期刊名称：AQUACULTURE （ 影响因子：4.5； 五年影响因子：4.6 ）

ISSN： 0044-8486

年卷期： 2023 年 570 卷

页码：

收录情况： SCI

摘要： A 95-day feeding experiment was designed to evaluate the effects of fish meal with enzyme-hydrolysed intestinal mucosa protein (EMP) replacement of 0% (EMP0), 6.7% (EMP6.7), 13.3% (EMP13.3), 20.0% (EMP20.0), and 26.7% (EMP26.7) in Micropterus salmoides (M. salmoides). No negative effects were observed in the diet in which fish meal was replaced by EMP with 6.7%-13.3%, according to the growth and feed utilization indicators. Interestingly, food intake (FI) increased significantly with the replacement level of EMP. Transcriptome analysis showed that compared with the brains of M. salmoides fed the EMP0 diet, the brains of M. salmoides fed a low-fish-meal diet (EMP26.7) exhibited 1405 differentially expressed genes (DEGs), including 829 upregulated DEGs and 576 downregulated DEGs. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment indicated that EMP26.7 promoted the feeding of M. salmoides primarily by upregulating orexigenic factors and downregulating anorexigenic factors. The ingestion-related factors pomc, cck, and lpar1 were significantly correlated. Further-more, GO and KEGG enrichment showed that long-term consumption of a low-fish-meal diet (EMP26.7) significantly affected oxidative stress and inflammation-related factors in the brain. Antioxidant indicators, including SOD, GSH, and T-AOC, were significantly downregulated, and MDA content was significantly upre-gulated in the brains of fish fed a low-fish-meal diet (EMP26.7 diet). SOD, GSH, and T-AOC were significantly correlated with epi-1 and negatively correlated with tlr13 and nod1. Negative relationships were found between MDA and epi-1, and positive relationships were found between nod1 and aqp3. In conclusion, the EMP could replace 6.7%-13.3% fish meal in the diet of M. salmoides without adversely affecting growth performance. The feeding response of M. salmoides to a low-fish-meal diet is to increase FI through the related feeding regulators in neuroactive ligand-receptor interaction, and a low-fish-meal diet can cause oxidative stress in the brain.

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