文献类型： 外文期刊

作者： Ye, Nan ¹ ; Bian, Jia-Shen ¹ ; Zhou, Bai-Hui ¹ ; Yong, Ling-Tao ¹ ; Yang, Ting ¹ ; Wang, Nan ¹ ; Dong, Yuan-Yuan ¹ ; Liu, Wei-Can ¹ ; Wang, Fa-Wei ¹ ; Lv, Hai-Yan ³ ; Li, Xiao-Wei ¹ ;

作者机构： 1.Jilin Agr Univ, Coll Life Sci, Changchun 130118, Peoples R China

2.Jilin Acad Agr Sci, Inst Crop Germplasm Resources, Northeast Agr Res Ctr China, Gongzhuling 136100, Peoples R China

3.Jilin Agr Univ, Coll Hort, Changchun 130118, Peoples R China

关键词： GmSNF4; soybean; salt-alkali stress; transgenic plants

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 14 期

页码：

收录情况： SCI

摘要： In order to mitigate the reduction in soybean yield caused by soil salinization, a soybean gene, GmSNF4, which promotes plant tolerance to salt-alkali stress, was identified in this study. The STRING database was used to predict the interaction between GmSNF4 and GmPKS4. The GmPKS4 gene was experimentally shown to be involved in salt-alkali stress tolerance. Firstly, the yeast two-hybrid technique and bimolecular fluorescence complementation (BiFC) technique were used to confirm the interaction between GmSNF4 and GmPKS4: the AMPK-CBM-CBS1 conserved domain was thereby determined to be the region of the GmSNF4 protein involved in the interaction. Secondly, the GmSNF4 gene was induced by salt-alkali stress according to qRT-PCR analysis, and the GmSNF4 protein was localized in the nucleus and cytoplasm. Finally, analysis of GmSNF4's role in resistance to salt-alkali stress in transgenic soybean plants showed that transgenic lines had better phenotypic, physiological, and stress-related gene expression than non-transgenic soybeans. Thus, GmSNF4 may play a significant role in plant salt-alkali stress tolerance.