A novel self-enhanced electrochemiluminescent aptamer sensor based on ternary nanocomposite PEI/RuSi-MWCNTs for the detection of profenofos residues in vegetables
文献类型： 外文期刊
作者： He, Zhenying 1 ; Wang, Haifang 5 ; Liu, Wenzheng 1 ; Sun, Jiashuai 1 ; Huang, Jingcheng 1 ; Han, Jie 1 ; Li, Baoxin 1 ; Xu, Rui 1 ; Zhang, Yuhao 1 ; Hua, Jin 1 ; Guo, Yemin 1 ; Lu, Fangyuan 1 ; Shi, Ce 2 ;
作者机构： 1.Shandong Univ Technol, Sch Agr Engn & Food Sci, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Informat Technol Res Ctr, Beijing 100097, Peoples R China
3.Zibo City Key Lab Agr Prod Safety Traceabil, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
4.Shandong Prov Engn Res Ctr Vegetable Safety & Qual, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
5.Beijing Univ Chinese Med, Dongzhimen Hosp, Beijing 100700, Peoples R China
关键词： Electrochemiluminescence; Ru(bpy)32+; Multi-walled carbon nanotubes; Profenofos; Aptasensor
期刊名称：HELIYON （ 影响因子：4.0； 五年影响因子：4.1 ）
ISSN：
年卷期： 2024 年 10 卷 3 期
页码：
收录情况： SCI
摘要： In this work, a novel ternary nanocomposite of PEI/RuSi-MWCNTs was designed and synthesized for the first time, which an ultrasensitive and self -enhanced electrochemiluminescent (ECL) aptasensor was developed for the detection of profenofos residues in vegetables. The selfenhanced complex PEI-Ru (II) enhanced the emission and stability of ECL, and the multiwalled carbon nanotubes (MWCNTs) acted as an excellent carrier and signal amplification. The PEI/RuSi-MWCNTs were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM) and energy dispersive spectrometer (EDS). The incorporation of gold nanoparticles (AuNPs) improved the performance of the sensor and provided a platform for the immobilization of the aptamer. The results of the experiment showed that the presence of profenofos significantly suppressed the electrochemiluminescence intensity of the sensor. The detection sensitivity of the aptamer sensor was in the range of 1 x 10-2 to 1 x 103 ng/mL. Under optimal conditions, the limit of detection (LOD) of the sensor for profenofos was 1.482 x 10-3 ng/mL. The sensor had excellent stability, reproducibility and specificity. The recoveries of the sensor ranged from 92.29 % to 106.47 % in real sample tests.
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