Sensory directed analysis for key odor-active compounds in by-products flavorings enhanced by enzymatic hydrolysis and Maillard reaction
文献类型： 外文期刊
作者： Han, Xiaomo 1 ; Gao, Xing 1 ; Yu, Mingguang 2 ; Xu, Rui 1 ; Song, Huanlu 1 ; Pan, Wenqing 3 ; Chen, Wanying 3 ; Xiong, Wen 3 ; Yang, Li 3 ; Huang, Zijian 4 ;
作者机构： 1.Beijing Technol & Business Univ, Sch Food & Hlth, Lab Mol Sensory Sci, Beijing 100048, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Inst Qual Stand & Testing Technol, Beijing 100097, Peoples R China
3.Prov Jiapinjiawei Biotechnol Co Ltd, Changde 415401, Hunan, Peoples R China
4.Hunan Agr Univ, Sch Life Sci, Changsha 410128, Peoples R China
关键词： Chicken bone; Maillard reaction; GC xGC-O-MS; Key odor-active compounds; Flavor enhancement
期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.6； 五年影响因子：4.6 ）
ISSN： 0889-1575
年卷期： 2025 年 142 卷
页码：
收录情况： SCI
摘要： This study explores the enhancement of chicken bone flavor through enzymatic hydrolysis and the Maillard reaction, providing insights into the formation and characterization of key aroma compounds. Chicken bone extract was hydrolyzed using ProtamexTM and FlavourzymeTM to obtain chicken bone enzymatic hydrolysate (CBEE), which was subjected to the Maillard reaction to produce Maillard reaction chicken flavoring (MRCF). The volatile compounds in CBEE and MRCF were comprehensively analyzed using solvent-assisted flavor evaporation (SAFE) and solid-phase microextraction (SPME) combined with two-dimensional gas chromatography-olfactometry-mass spectrometry (GCxGC-O-MS). A total of 40 odor active compounds were identified and their odor activity values (OAV) were calculated. Among them, 11 compounds contributed significantly to the overall aroma (OAV >= 1). Key contributors, including 2-methyl-3-furanethiol, furaneol, 5-(2-hydroxyethyl)4-methylthiazole, and maltol, were confirmed through aroma recombination and omission experiments. The results highlight that the Maillard reaction significantly enhances the richness and complexity of the meaty and roasted aromas, driven primarily by the increased levels of sulfur-containing and heterocyclic compounds. This research provides a scientific foundation for the industrial development of meat flavorings from chicken bone byproducts and offers valuable guidance for flavor optimization.
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