您好，欢迎访问山西省农业科学院 机构知识库！
山西省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Expression of alfalfa antifungal peptide gene and enhance of resistance to Verticillium dahliae in upland cotton

文献类型： 外文期刊

作者： Zhang, Haiping 1 ; Wang, Xuede 1 ; Shao, Mingyan 1 ; Yuan, Shuna 1 ; Ni, Mi 1 ;

作者机构： 1.Zhejiang Univ, Coll Agr & Biotechnol, Dept Agron, Hangzhou 310029, Peoples R China

2.Shanxi Acad Agr Sci, Inst Crop Germplasm Resources, Taiyuan, Peoples R China

关键词： Agrobacterium tumefaciens;cotton;disease-resistance;transformation;Verticillium dahliae

期刊名称：ACTA AGRICULTURAE SCANDINAVICA SECTION B-SOIL AND PLANT SCIENCE （ 影响因子：1.694； 五年影响因子：1.568 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In order to increase cotton plant resistance to Verticillium dahliae (V. dahliae), an alfalfa antifungal peptide gene (alfAFP), directed by CaMV35S promoter in recombinant binary vector pCAMBIA1301-alf, was delivered into an upland cotton cultivar 5983 (Gossypium hirsutum L.) via an Agrobacterium-mediated hypocotyl system. Molecular analysis confirmed the integration and transcripts of the alfAFP gene in the genome of 12 transformants. In vitro assays showed that crude leaf extracts from transformants was able to significantly (p<0.01) inhibit the growth and proliferation of V. dahliae compared to the extracts from control plants. In vivo assays with fungal pathogens showed that transformants displayed a significant reduction in their disease symptoms compared to control plants. These results suggest that the alfAFP gene was expressed in the upland cotton and its product of gene expression was active in the growth inhibition of V. dahliae.

  • 相关文献

[1]Regeneration of Chinese cabbage transgenic plants expressing antibacterial peptide gene and cowpea trypsin inhibitor gene. Zhao, Junliang,Liang, Aihua,Zhu, Zhen,Tang, Yixiong. 2006

[2]Agrobacterium-mediated co-transformation of multiple genes in upland cotton. Li, Fei-Fei,Wu, Shen-Jie,Chen, Tian-Zi,Zhang, Jie,Wang, Hai-Hai,Guo, Wang-Zhen,Zhang, Tian-Zhen,Wu, Shen-Jie. 2009

[3]Overexpression of a foxtail millet Acetyl-CoA carboxylase gene in maize increases sethoxydim resistance and oil content. Dong, Zhigang,Zhao, Huji,He, Junguang,Huai, Junling,Lin, Heng,Wang, Guoying,Dong, Zhigang,Zheng, Jun,Liu, Yunjun,Wang, Guoying. 2011

[4]Effects of Soil Salinity and Plant Density on Yield and Leaf Senescence of Field-Grown Cotton. Zhang, H. J.,Dong, H. Z.,Li, W. J.,Zhang, D. M.,Zhang, H. J.. 2012

[5]Review of the technology for high-yielding and efficient cotton cultivation in the northwest inland cotton-growing region of China. Lu Feng,Jianlong Dai,Dong, Hezhong,Liwen Tian,Huijun Zhang,Weijiang Li,Hezhong Dong.

[6]Pistil drip following pollination: a simple in planta Agrobacterium-mediated transformation in cotton. Chen TianZi,Wu ShenJie,Zhao Jun,Guo WangZhen,Zhang TianZhen,Wu ShenJie.

[7]Marker-assisted selection of Verticillium wilt resistance in progeny populations of upland cotton derived from mass selection-mass crossing. Li, Cheng-Qi,Liu, Guan-Shen,Zhao, Hai-Hong,Wang, Li-Juan,Zhang, Xiao-Fei,Liu, Ying,Zhou, Wan-Yang,Wang, Qing-Lian,Yang, Liu-Liu,Li, Peng-Bo.

[8]Effect of phytohormones on fiber initiation of cotton ovule. Zhang, Haiping,Shao, Mingyan,Yuan, Shuna,Wang, Xuede,Hua, Shuijin,Zhang, Haiping,Qiao, Zhijun.

[9]Effectiveness of thiamethoxam and imidacloprid seed treatments against Bemisia tabaci (Hemiptera: Aleyrodidae) on cotton. Liu, Tong-Xian,Zhang, Liping,Zhang, Yongmei,Liu, Tong-Xian,Greenberg, Shoil M.,Zhang, Liping.

[10]Nitrous oxide and nitric oxide emissions from an irrigated cotton field in Northern China. Liu, Chunyan,Zheng, Xunhua,Zhou, Zaixing,Han, Shenghui,Wang, Yinghong,Wang, Kai,Liang, Wangguo,Li, Ming,Chen, Deli,Yang, Zhiping.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山西省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台