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A 796 bp PsPR10 gene promoter fragment increased root-specific expression of the GUS reporter gene under the abiotic stresses and signal molecules in tobacco

文献类型： 外文期刊

作者： Xu, Xiangbin 1 ; Guo, Sai 1 ; Chen, Kai 1 ; Song, Hongmiao 2 ; Liu, Junjun 1 ; Guo, Longbiao 4 ; Qian, Qian 4 ; Wang, Hui 1 ;

作者机构： 1.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 310036, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou 310021, Zhejiang, Peoples R China

3.Nat Resources Canada, Pacific Forestry Ctr, Canadian Forest Serv, Victoria, BC V8Z 1M5, Canada

4.China Natl Rice Res Inst, Hangzhou 310006, Zhejiang, Peoples R China

关键词： Pinus strobus.;GUS;PR10 gene;Promoter;Root-specific expression;Internet resource.

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

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收录情况： SCI

摘要： A 1681 bp PsPR10 promoter was isolated from Pinus strobus and a series of 5o-deletions were fused to the o-glucuronidase (GUS) reporter gene and introduced into tobacco. GUS activity in P796 (796 to +69) construct transgenic plant roots was similar with that of P1681 and higher than those of the P513 (513 to +69) and P323 (323 to +69) transgenic plants. Moreover, the abiotic stresses of NaCl, PEG 6000 and mannitol, and salicylic acid (SA), abscisic acid (ABA) and jasmonic acid (JA) induced higher GUS activity in the roots of P796 transgenic tobacco. This study provides a potential inducible root-specific promoter for transgenic plants.

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