Fine Mapping and Cloning of a qRA2 Affect the Ratooning Ability in Rice (Oryza sativa L.)
文献类型： 外文期刊
作者： He, Niqing 1 ; Huang, Fenghuang 1 ; Yang, Dewei 1 ;
作者机构： 1.Fujian Acad Agr Sci, Rice Res Inst, Fujian High Qual Rice Res & Dev Ctr, Fuzhou 350018, Peoples R China
关键词： rice (Oryza sativa L; subsp; indica); gene mapping; gene cloning; ratooning ability; near-isogenic lines
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN：
年卷期： 2023 年 24 卷 2 期
页码：
收录情况： SCI
摘要： Ratooning ability is a key factor that influences the ratoon rice yield in areas where light and temperature are not sufficient for second-season rice. Near-isogenic lines (NILs) are the most powerful tools for the detection and precise mapping of quantitative trait loci (QTLs). In this study, using 176 NILs, we identified a novel QTL for ratooning ability in NIL128. First, we mapped the QTL between the markers Indel12-29 and Indel12-31, which encompass a region of 233 kb. The rice genome annotation indicated the existence of three candidate genes in this region that may be related to ratooning ability. Through gene prediction and cDNA sequencing, we speculated that the target gene of ratooning ability is LOC_Os02g51930 which encodes cytokinin glucosyl transferases (CGTs), hereafter named qRA2. Further analysis showed that qra2 was a 1-bp substitution in the first exon in NIL128, which resulted in the premature termination of qRA2. The results of the knockdown experiment showed that the Jiafuzhan knockdown mutants exhibited the ratooning ability phenotype of NIL128. Interestingly, the qRA2 gene was found to improve ratooning ability without affecting major agronomic traits. These results will help us better understand the genetic basis of rice ratooning ability and provide a valuable gene resource for breeding strong ratoon rice varieties.
- 相关文献
作者其他论文 更多>>
-
Nucleotide-binding leucine-rich repeat receptor homologs Pib and PibH8 interact and contribute to immunity in rice
作者：Wang, Zhanchun;Zhong, Guitao;Li, Shengping;Wang, Wei;Tang, Dingzhong;Yang, Dewei;Zhong, Guitao;Li, Shengping
关键词：
-
Alternative polyadenylation profiles of susceptible and resistant rice (Oryza sativa L.) in response to bacterial leaf blight using RNA-seq
作者：Liu, Shaochun;Luo, Shuqi;Huang, Junying;Jiang, Xinlei;Yu, Shangwei;Fu, Junru;Zhou, Dahu;Chen, Xiaorong;He, Haohua;Fu, Haihui;Yang, Dewei
关键词：Alternative polyadenylation; Rice bacterial leaf blight; Xanthomonas oryzae pv. oryzae; RNA-seq; Rice (Oryza sativa L.)
-
Identification fi cation of the RRM1 gene family in rice ( Oryza sativa) ) and its response to rice blast
作者：Jiang, Xinlei;Yu, Shangwei;Huang, Yuhan;Huang, Junying;Liu, Shaochun;Yang, Dewei;He, Haohua;Fu, Haihui;Yang, Dewei
关键词：Rice; Gene family; Rice blast; Bioinformatics
-
Receptor-like kinases OsRLK902-1 and OsRLK902-2 form immune complexes with OsRLCK185 to regulate rice blast resistance
作者：Liang, Jiahui;Lu, Ling;Zhou, Houli;Fang, Jianbo;Hou, Hongna;Chen, Lizhe;Cao, Chang;Diao, Zhijuan;Tang, Dingzhong;Li, Shengping;Zhao, Yaofei;Yang, Dewei;Diao, Zhijuan;Tang, Dingzhong;Li, Shengping;Li, Shengping
关键词：Disease resistance; M. oryzae; OsRLCK185; OsRLK902; receptor-like kinase; rice; rice blast
-
The Genetic Mechanism of the Immune Response to the Rice False Smut (RFS) Fungus Ustilaginoidea virens
作者：Yang, Dewei;He, Niqing;Huang, Fenghuang;Jin, Yidan;Li, Shengping;Li, Shengping
关键词：rice; rice false smut; research progress; genetic mechanisms; QTL; resistance protein
-
SPR9 encodes a 60 S ribosomal protein that modulates panicle spreading and affects resistance to false smut in rice (Oryza sativa. L)
作者：He, Niqing;Huang, Fenghuang;Lu, Libin;Wang, Xun;Li, Qingshun Q. Q.;Yang, Dewei;Li, Qingshun Q. Q.
关键词：Rice (Oryza sativa L; ); Spreading panicle mutant; Gene cloning; Rice false smut (RFS)
-
Fine Mapping and Cloning of a Major QTL qph12, Which Simultaneously Affects the Plant Height, Panicle Length, Spikelet Number and Yield in Rice (Oryza sativa L.)
作者：He, Niqing;Zhan, Guanping;Huang, Fenghuang;Yang, Dewei;Zhan, Guanping;Abou-Elwafa, Salah Fatouh
关键词：rice (Oryza sativa L; subsp; indica); gene mapping; gene cloning; auxin; near-isogenic lines
