文献类型： 外文期刊

作者： Liang, Jiahui ¹ ; Lu, Ling ¹ ; Zhou, Houli ¹ ; Fang, Jianbo ¹ ; Zhao, Yaofei ² ; Hou, Hongna ¹ ; Chen, Lizhe ¹ ; Cao, Chang ¹ ; Yang, Dewei ³ ; Diao, Zhijuan ¹ ; Tang, Dingzhong ¹ ; Li, Shengping ¹ ;

作者机构： 1.Fujian Agr & Forestry Univ, Plant Immun Ctr, State Key Lab Ecol Control Fujian Taiwan Crop Pest, Key Lab,Minist Educ Genet Breeding & Multiple Util, Fuzhou 350002, Peoples R China

2.Shanxi Agr Univ, Coll Agr, Taigu 030801, Shanxi, Peoples R China

3.Fujian Acad Agr Sci, Rice Res Inst, Fuzhou 350019, Peoples R China

4.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Peoples R China

5.Fujian Agr & Forestry Univ, Ministerial & Prov Joint Innovat Ctr Safety Prod C, Fuzhou 350002, Peoples R China

6.Fujian Agr & Forestry Univ, Fujian Prov Key Lab Crop Breeding Design, Fuzhou, Peoples R China

关键词： Disease resistance; M. oryzae; OsRLCK185; OsRLK902; receptor-like kinase; rice; rice blast

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）

ISSN： 0022-0957

年卷期： 2024 年 75 卷 5 期

页码：

收录情况： SCI

摘要： Receptor-like kinases (RLKs) are major regulators of the plant immune response and play important roles in the perception and transmission of immune signals. RECEPTOR LIKE KINASE 902 (RLK902) is at the key node in leucine-rich repeat receptor-like kinase interaction networks and positively regulates resistance to the bacterial pathogen Pseudomonas syringae in Arabidopsis. However, the function of RLK902 in fungal disease resistance remains obscure. In this study, we found that the expression levels of OsRLK902-1 and OsRLK902-2, encoding two orthologues of RLK902 in rice, were induced by Magnaporthe oryzae, chitin, and flg22 treatment. osrlk902-1 and osrlk902-2 knockout mutants displayed enhanced susceptibility to M. oryzae. Interestingly, the osrlk902-1 rlk902-2 double mutant exhibited similar disease susceptibility, hydrogen peroxide production, and callose deposition to the two single mutants. Further investigation showed that OsRLK902-1 interacts with and stabilizes OsRLK902-2. The two OsRLKs form a complex with OsRLCK185, a key regulator in chitin-triggered immunity, and stabilize it. Taken together, our data demonstrate that OsRLK902-1 and OsRLK902-2, as well as OsRLCK185 function together in regulating disease resistance to M. oryzae in rice. Receptor-like kinases OsRLK902-1 and OsRLK902-2 are required for rice blast resistance by forming immune complexes with OsRLCK185 and stabilizing OsRLCK185, a key regulator in chitin-triggered immunity.