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Importance of OsRac1 in Signalling of Pigm-1 Mediated Resistance to Rice Blast Disease

文献类型： 外文期刊

作者： Yang, Dewei 1 ; He, Niqing 1 ; Huang, Fenghuang 1 ; Chen, Jialin 2 ; Yu, Minxiang 1 ; Jin, Yidan 2 ; Lin, Shaojun 1 ; Li, Shengping 2 ;

作者机构： 1.Fujian Acad Agr Sci, Inst Rice, Fuzhou 350018, Peoples R China

2.Fujian Agr & Forestry Univ, Coll Agr, Plant Immun Ctr, Fuzhou 350002, Peoples R China

3.Fujian Agr & Forestry Univ, Fujian Prov Key Lab Crop Breeding Design, Fuzhou 350002, Peoples R China

关键词： Pigm-1; OsRac1; rice blast resistance; signal transduction; molecular breeding

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 2 期

页码：

收录情况： SCI

摘要： In rice, leucine-rich repeat nucleotide-binding site (NLR) proteins are pivotal immune receptors in combating Magnaporthe oryzae-triggered rice blast. However, the precise molecular mechanism underlying how NLR proteins regulate downstream signalling remains elusive due to the lack of knowledge regarding their direct downstream targets. The NLR protein Pigm-1 was cloned from Shuangkang 77009 in our laboratory. This study shows that the nucleotide-binding site (NBS) domain of Pigm-1 facilitates its binding to and activation of OsRac1 while the coiled-coil (CC) domain enables its binding to and activation of RAI1, ultimately inducing cell death. At the same time, after knocking out OsRac1 in the background of Shuangkang 77009 containing Pigm-1, two knockout lines showed susceptibility to rice blast. This study reveals OsRac1, a GTPase, as a signalling molecule involved in Pigm-1-mediated blast resistance, suggesting its potential as a common downstream effector of rice NLR proteins. Additionally, a transcriptional activator, RAI1, acts as an essential Pigm-1 interactor for blast resistance. Furthermore, a novel material 9311(Pigm-1) was prepared by using two-line restorer line 9311 as receptor and Shuangkang 77009 as donor with molecular marker-assisted technology, which improved blast resistance and yield. This research demonstrates that molecular marker-assisted selection technology enhances both resistance and yield in the crucial two-line restorer 9311(Pigm-1). This study offers crucial insights into how Pigm-1 protein activates downstream molecules and serves as a valuable reference for the molecular breeding of rice blast resistance genes, particularly Pigm-1.

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