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Simultaneous estimation of vinpocetine and its metabolite, apovincaminic acid, in beagle plasma by LC-MS-MS

文献类型： 外文期刊

作者： Yang, Yuan 1 ; Lou, Kun 2 ; Gong, Wei 2 ; Mei, Xing-Guo 2 ; Zhang, Kai-Chun 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 100093, Peoples R China

2.Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China

3.CSPC Zhongqi Pharmaceut Technol Co Ltd, Shijiazhuang 050051, Hebei, Peoples R China

关键词： Apovincaminic acid;Column liquid chromatography-mass spectrometry;Multiple reaction monitoring;Vinpocetine

期刊名称：CHROMATOGRAPHIA （ 影响因子：2.044； 五年影响因子：1.725 ）

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收录情况： SCI

摘要： A method was developed to determine vinpocetine and its metabolite, apovincaminic acid, in beagle plasma by LC-MS-MS. After protein precipitation with methanol, the supernatant of the sample was concentrated and injected into an Agilent Zorbax XDB-C_(18) column. The sample was separated by a mobile phase consisting of acetonitrile and 0.2% formic acid solution, and the reading was determined on an Agilent 6410 Triple Quad Tandem mass spectrometer in multiple reaction monitoring mode with the following transitions: m/z 351.5 → 280.2/266.3 for vinpocetine, 323.2 → 236.1/280.2 for apovincaminic acid, and 411.2 → 191.1 for the internal standard. The intra- and inter-day variances were less than 15% (RSD%), and average recoveries were higher than 80%. The linearity ranges (LR) between 0.1 and 20.0 ng mL~(-1) for vinpocetine (r ~2 = 0.9980) and between 1.0 and 200.0 ng mL ~(-1) for apovincaminic acid (r ~2 = 0.9995) were established. In summary, this method is sensitive, specific, and appropriate for in vivo study of various dosage forms of vinpocetine.

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