您好，欢迎访问河北省农林科学院 机构知识库！
河北省农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Metabolomics and transcriptomics provide insights into the flavonoid biosynthesis pathway in the roots of developing Aster tataricus

文献类型： 外文期刊

作者： Jia, Kaixuan 1 ; Zhang, Xiaoling 1 ; Meng, Yijiang 2 ; Liu, Shuqi 1 ; Liu, Xiaoqing 1 ; Yang, Taixin 1 ; Wen, Chunxiu 4 ; Liu, Lingdi 4 ; Ge, Shujun 1 ;

作者机构： 1.Hebei Agr Univ, Coll Agron, West Campus, Baoding 071000, Hebei, Peoples R China

2.Minist Educ, Key Lab Crop Germplasm Resources Res & Utilizat No, Baoding 071000, Peoples R China

3.Hebei Agr Univ, Coll Life Sci, Baoding 071000, Peoples R China

4.Hebei Acad Agr & Forestry Sci, Inst Cash Crops, Med Plant Res Ctr West, Nongke Rd,Xiyuan St, Shijiazhuang 050000, Hebei, Peoples R China

关键词： Aster tataricus; Differentially expressed genes; Flavonoids; Metabolite; Transcriptome

期刊名称：JOURNAL OF PLANT RESEARCH （ 影响因子：2.8； 五年影响因子：3.0 ）

ISSN： 0918-9440

年卷期： 2023 年 136 卷 1 期

页码：

收录情况： SCI

摘要： Aster tataricus (L.) is an important medicinal plant in China. Its roots are rich in flavonoids, the main medicinal components. However, the molecular basis of flavonoid biosynthesis in the roots of A. tataricus remains unclear. In this study, the content of total flavonoid of A. tataricus roots at different developmental stages was measured first, and the results showed that the content of total flavonoid gradually decreased from September to November, which may be caused by the stagnation of A. tataricus growth due to the decrease in temperature after September. Then, an integrated analysis of transcriptome and metabolome was conducted on five developing stages of A. tataricus roots to identify flavonoid compositions and potential genes involved in flavonoid biosynthesis. A total of 80 flavonoid metabolites, of which 75% were flavonols and flavonoids, were identified in metabolomic analyses, among which isorhamnetin, kaempferol, quercetin, and myricetin were the main skeletons of these flavonoids. Cluster analysis divided these 80 flavonoids into 3 clusters. The compounds in cluster I mainly accumulated in S1, S3, and S5. In cluster II, the relative content of the flavonoid metabolites showed an upward trend from S2 to S4. In cluster III, the flavonoids decreased from S1 to S5. A total of 129 structural genes, including 43 PAL, 23 4CL, 9 C4H, 4 CHS, 18 CHI, 3 F3H, 5 F3'H, 1 F3 & PRIME;5 & PRIME;H, 21 FLS, and 2 FSII, and 65 transcription factors, including 22 AP2/ERF, 7 bHLH, 5 bZIP, 8 MYB, 11 NAC, and 12 WRKY, showed significant correlation with total flavonoid content. Eighteen genes (7 4CL, 5 C4H, 2 CHI, 1 F3H, and 3 FLS) and 30 genes (5 PAL, 9 4CL, 1 C4H, 2 CHI, 1 F3H, 1 DFR, 7 3AT, 1 BZ1, and 3 UGT79B1) were identified as key structural genes for kaempferol and anthocyanins biosynthesis, respectively. Our study provides valuable information for understanding the mechanism of flavonoid biosynthesis in A. tataricus root.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河北省农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台