Spatial Mapping of Bioactive Metabolites in the Roots of Three Bupleurum Species by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
文献类型： 外文期刊
作者： Han, Xiaowei 1 ; Ma, Donglai 1 ; Wang, Jiemin 1 ; Pei, Lin 2 ; Liu, Lingdi 3 ; Shi, Weihong 1 ; Rong, Zhengpu 1 ; Wang, Xiaoyuan 1 ; Zhang, Ye 1 ; Zheng, Yuguang 1 ; Sun, Huigai 1 ;
作者机构： 1.Hebei Univ Chinese Med, Coll Pharm, Shijiazhuang 050200, Peoples R China
2.Hebei Acad Tradit Chinese Med, Shijiazhuang 050031, Peoples R China
3.Hebei Acad Agr & Forestry Sci, Inst Cash Crops, Shijiazhuang 050051, Peoples R China
关键词： Bupleurum; saikosaponins; non-saikosaponins; saikosaponin synthesis pathway compounds; MALDI-MSI; OPLS-DA; HPLC
期刊名称：MOLECULES （ 影响因子：4.6； 五年影响因子：5.0 ）
ISSN：
年卷期： 2024 年 29 卷 16 期
页码：
收录情况： SCI
摘要： Bupleurum is a kind of medicinal plant that has made a great contribution to human health because of the presence of bioactive metabolites: Bupleurum saikosaponins and flavonoids. Despite their importance, it has been a challenge to visually characterize the spatial distribution of these metabolites in situ within the plant tissue, which is essential for assessing the quality of Bupleurum. The development of a new technology to identify and evaluate the quality of medicinal plants is therefore necessary. Here, the spatial distribution and quality characteristics of metabolites of three Bupleurum species: Bupleurum smithii (BS), Bupleurum marginatum var. stenophyllum (BM), and Bupleurum chinense (BC) were characterized by Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). Twenty-nine metabolites, including saikosaponins, non-saikosaponins, and compounds from the saikosaponin synthesis pathway, were characterized. Some of these were successfully localized and visualized in the transverse section of roots. In these Bupleurum species, twelve saikosaponins, five non-saikosaponins, and five saikosaponin synthesis pathway compounds were detected. Twenty-two major influencing components, which exhibit higher ion intensities in higher quality samples, were identified as potential quality markers of Bupleurum. The final outcome indicates that BC has superior quality compared to BS and BM. MALDI-MSI has effectively distinguished the quality of these Bupleurum species, providing an intuitive and effective marker for the quality control of medicinal plants.
- 相关文献
作者其他论文 更多>>
-
Dynamic Metabolite Profile Changes in Semen Ziziphi Spinosae During Ripening
作者：Tian, Xi;Tian, Wei;Liu, Lingdi;Jiang, Tao;Zhang, Sujun;Gu, Liqiang;Li, Qiang
关键词：different harvest times; metabolite;
Semen Ziziphi Spinosae ; UPLC-Q Trap-MS/MS -
The pan-plastome reveals the genetic diversity and genetic divergence of Forsythia suspensa (Oleaceae) from the maternal inheritance perspective
作者：Cao, Linlin;Guo, Chang;Yan, Pengpeng;Liu, Kangjia;Li, Yuhan;Dong, Wenpan;Sun, Jiahui;Wang, Yiheng;Wang, Sheng;Wu, Nan;Liu, Lingdi;Wang, Sheng
关键词：Genetic divergence; Geographic isolation; Weeping forsythia; Pan-plastomes
-
Integrated Data Reveal the Flavonoid Biosynthesis Metabolic Pathway in Semen Ziziphi Spinosae Using Transcriptomic and Metabolomic
作者：Tian, Wei;Liu, Lingdi;Wen, Chunxiu;Jiang, Tao;Song, Zhongxing;Tang, Zhishu
关键词：Spinosin; secondary metabolites; transcriptomic; metabolomic; gene expression
-
Metabolomics and transcriptomics provide insights into the flavonoid biosynthesis pathway in the roots of developing Aster tataricus
作者：Jia, Kaixuan;Zhang, Xiaoling;Liu, Shuqi;Liu, Xiaoqing;Yang, Taixin;Ge, Shujun;Jia, Kaixuan;Zhang, Xiaoling;Meng, Yijiang;Liu, Shuqi;Liu, Xiaoqing;Yang, Taixin;Ge, Shujun;Meng, Yijiang;Wen, Chunxiu;Liu, Lingdi
关键词：Aster tataricus; Differentially expressed genes; Flavonoids; Metabolite; Transcriptome
-
Metabolomics Analysis of Metabolites in Forsythia suspense Fruit Using UPLC/ESI-Q TRAP-MS/MS
作者：Liu, Lingdi;Wen, Chunxiu;Tian, Wei;Xie, Xiaoliang;Wen, Saiqun;Jiang, Tao
关键词：Forsythiae Fructus; traditional Chinese medicine; UPLC/ESI-Q TRAP-MS/MS; component; metabolite
-
Metabolome analysis of genus Forsythia related constituents in Forsythia suspensa leaves and fruits using UPLC-ESI-QQQ-MS/MS technique
作者：Liu, Lingdi;Wen, Chunxiu;Jiang, Tao;Tian, Wei;Xie, Xiaoliang;Lu, Ruike;Feng, Jiaxing;Jin, Aihong;Wen, Saiqun;Liu, Lingdi;Wen, Chunxiu;Jiang, Tao;Tian, Wei;Xie, Xiaoliang;Lu, Ruike;Feng, Jiaxing;Jin, Aihong;Wen, Saiqun;Sun, Yu;Wei, Wei;Cui, Xusheng
关键词：
-
Integrated transcriptomic and metabolomic data reveal the flavonoid biosynthesis metabolic pathway in Perilla frutescens (L.) leaves
作者：Jiang, Tao;Liu, Lingdi;Tian, Wei;Xie, Xiaoliang;Wen, Saiqun;Wen, Chunxiu;Guo, Kunyuan
关键词：
