Integrated Data Reveal the Flavonoid Biosynthesis Metabolic Pathway in Semen Ziziphi Spinosae Using Transcriptomic and Metabolomic
文献类型： 外文期刊
作者： Tian, Wei 1 ; Liu, Lingdi 1 ; Song, Zhongxing 2 ; Tang, Zhishu 2 ; Wen, Chunxiu 1 ; Jiang, Tao 1 ;
作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Cash Crops, Shijiazhuang 050051, Hebei, Peoples R China
2.Shaanxi Univ Chinese Med, State Key Lab Res & Dev Characterist Qin Med Reso, Xianyang 712046, Peoples R China
关键词： Spinosin; secondary metabolites; transcriptomic; metabolomic; gene expression
期刊名称：PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：1.7； 五年影响因子：1.4 ）
ISSN： 0031-9457
年卷期： 2023 年 92 卷 2 期
页码：
收录情况： SCI
摘要： The extract from Semen Ziziphi Spinosae (SZS) contains many secondary metabolites (flavonoids, alkaloids, and terpenoids), widely reported for their high medicinal value. In order to know the number and type of flavonoids and the regulating mechanism of flavonoid biosynthesis during the growth and development of SZS, we identified and analyzed the flavonoid metabolites and genes in SZS using metabolomic and transcriptomic. A total of 13,232 differentially expressed genes and 83 flavonoid metabolites were identified at three different growth and development stages (T1, T2, and T3) of SZS. The main flavonoid metabolic components of SZS were cate-chin, L-epicatechin, (-)-epigallocatechin, (+)-gallocatechin, spinosin and its derivatives. A total of 53 unigenes encoding 15 enzymes were identified as candidate genes involved in flavonoid biosynthesis in SZS. Among, PAL, C4H, 4CL, CHS, CHI, FLS, FNS, ANS, ANR, LAR, and UFGT genes were all downregulated in T3 than in T1. The expression levels of these genes influence the accumulation of flavonoids in SZS. Our results should pro-vide valuable information for flavonoid metabolites and candidate genes involved in flavonoid biosynthesis on SZS
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