Screening and identification of female-specific DNA sequences in octaploid sturgeon using comparative genomics with high-throughput sequencing
文献类型： 外文期刊
作者： Ruan, Rui 1 ; Feng, Tong 2 ; Li, Ying 1 ; Yue, Huamei 1 ; Ye, Huan 1 ; Du, Hao 1 ; Liu, Qingyou 3 ; Ruan, Jue 2 ; Li, Chuang 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr & Rural Affairs China, Wuhan 430223, Peoples R China
2.Chinese Acad Agr Sci, Agr Genom Inst, Shenzhen 518120, Peoples R China
3.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530005, Peoples R China
关键词： Sex-specific DNA fragments; Complex genome; K-mers; Comparative genomics; Acipenseridae
期刊名称：GENOMICS （ 影响因子：5.736； 五年影响因子：4.939 ）
ISSN： 0888-7543
年卷期： 2021 年 113 卷 6 期
页码：
收录情况： SCI
摘要： In this study, six candidate female-specific DNA sequences of octaploid Amur sturgeon (Acipenser schrenckii) were identified using comparative genomic approaches with high-throughput sequencing data. Their specificity was confirmed by traditional PCR. Two of these sex-specific sequences were also validated as female-specific in other eight sturgeon species and two hybrid sturgeons. The identified female-specific DNA fragments suggest that the family Acipenseridae has a ZZ/ZW sex-determining system. However, one of the two DNA sequences has been deleted in some sturgeons such as Sterlet sturgeon (Acipenser ruthenus), Beluga (Huso huso) and Kaluga (H. dauricus). The difference of sex-specific sequences among sturgeons indicates that there are different sex specific regions among species of sturgeon. This study not only provided the sex-specific DNA sequences for management, conservation and studies of sex-determination mechanisms in sturgeons, but also confirmed the capability of the workflow to identify sex-specific DNA sequences in the polyploid species with complex genomes.
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