Electron Tomography Reveals Polyhedrin Binding and Existence of both Empty and Full Cytoplasmic Polyhedrosis Virus Particles inside Infectious Polyhedra
文献类型： 外文期刊
作者： Chen, Justin 1 ; Sun, Jingchen 3 ; Atanasov, Ivo 2 ; Ryazantsev, Sergey 2 ; Zhou, Z. Hong 1 ;
作者机构： 1.Univ Calif Los Angeles, Dept Microbiol Mol Genet & Immunol, Los Angeles, CA 90095 USA
2.Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA 90095 USA
3.S China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China
4.Univ Calif Los Angeles, David Geffen Sch Med, Dept Biol Chem, Los Angeles, CA 90095 USA
关键词： RNA;viral capsid;viral particles;foreign proteins;polyhedrin: B-spike;A-spike;RNA encapsidation;infectious polyhedra;viral protein packaging
期刊名称：JOURNAL OF VIROLOGY （ 影响因子：5.103； 五年影响因子：5.078 ）
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收录情况： SCI
摘要： Previous studies have described the structure of purified cytoplasmic polyhedrosis virus (CPV) and that of polyhedrin protein. However, how polyhedrin molecules embed CPV particles inside infectious polyhedra is not known. By using electron tomography, we show that CPV particles are occluded within the polyhedrin crystalline lattice with a random spatial distribution and interact with the polyhedrin protein through the A-spike rather than as previously thought through the B-spike. Furthermore, both full (with RNA) and empty (no RNA) capsids were found inside polyhedra, suggesting a spontaneous RNA encapsidating process for CPV assembly in vivo.
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