Dynamic deformylation of 5-formylcytosine and decarboxylation of 5-carboxylcytosine during differentiation of mouse embryonic stem cells into mouse neurons
文献类型： 外文期刊
作者： Feng, Yang 1 ; Tian, Yang-Qing 3 ; Zhao, Yong-Qiang 1 ; Chen, Sheng-Jun 1 ; Yuan, Bi-Feng 2 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Natl R&D Ctr Aquat Prod Proc, Key Lab Aquat Prod Proc,Minist Agr & Rural Affairs, Guangzhou 510300, Peoples R China
2.Wuhan Univ, Renmin Hosp, Res Ctr Publ Hlth, Sch Publ Hlth,Dept Occupat & Environm Hlth, Wuhan 430071, Peoples R China
3.Yunnan Agr Univ, Coll Vet Med, Kunming 650000, Peoples R China
关键词： Metabolic labeling; Mass spectrometry; DNA demethylation; Deformylation; Decarboxylation; C-C cleavage; Mouse embryonic stem cells; Differentiation
期刊名称：CHINESE CHEMICAL LETTERS （ 影响因子：9.4； 五年影响因子：7.3 ）
ISSN： 1001-8417
年卷期： 2024 年 35 卷 11 期
页码：
收录情况： SCI
摘要： Regulation of cell fate requires the establishment and erasure of 5-methylcytosine (5mC) in genomic DNA. The formation of 5mC is achieved by DNA cytosine methyltransferases (DNMTs), whereas the removal of 5mC can be accomplished by various pathways. Aside from ten-eleven translocation (TET)-mediated oxidation of 5mC followed by thymine DNA glycosylase (TDG)-initiated base excision repair (BER), the direct deformylation of 5-formylcytosine (5fC) and decarboxylation of 5-carboxylcytosine (5caC) have also been discovered as the novel DNA demethylation pathways. Although these novel demethylation pathways have been identified in stem cells and somatic cells, their precise roles in regulating cell fate remain unclear. Here, we differentiate mouse embryonic stem cells (mESCs) into mouse embryoid bodies (mEBs), followed by further differentiation into mouse neural stem cells (mNSCs) and finally into mouse neurons (mNeurons). During this sequential differentiation process, we employ probe molecules, namely 2' '-fluorinated 5-formylcytidine (F-5fC) and 2' '-fluorinated 5-carboxyldeoxycytidine (F-5caC), for metabolic labeling. The results of mass spectrometry (MS) analysis demonstrate the deformylation and decarboxylation activities are progressively decreased and increased respectively during differentiation process, and this opposite demethylation tendency is not associated with DNMTs and TETs. (c) 2024 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
- 相关文献
作者其他论文 更多>>
-
A Novel Strategy for the Detection of Semicarbazide in Crustaceans by Modified QuEChERS Coupled with Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry
作者：Wu, Siyuan;Chen, Shengjun;Wu, Siyuan;Feng, Yang;Chen, Shengjun;Zhao, Yongqiang;Li, Chunsheng;Deng, Jianchao;Wang, Di;Feng, Yang;Chen, Shengjun;Zhao, Yongqiang;Li, Chunsheng;Deng, Jianchao;Wang, Di
关键词：semicarbazide; crustaceans; shrimp; QuEChERS; HILIC-MS/MS;
Macrobrachium rosenbergii ; nitrofurazone; kinetic model -
Detection of amino thiols from fish samples by stable isotope chemical labelling coupled with ultra-performance liquid chromatography-tandem mass spectrometry
作者：Wu, Siyuan;Feng, Yang;Chen, Shengjun;Zhao, Yongqiang;Deng, Jianchao;Wang, Di;Feng, Yang;Chen, Shengjun;Zhao, Yongqiang;Deng, Jianchao;Wang, Di;Wu, Siyuan;Chen, Shengjun;Zhao, Yongqiang;Deng, Jianchao
关键词：Amino thiols; Liquid extraction; Iodoacetamide; Stable isotope labeling; UPLC-MS; Transportation stress
-
Integrated 4D label-free proteomics and data mining to elucidate the effects of thermal processing on crisp grass carp protein profiles
作者：Lin, Wan -ling;Liu, Ya-qun;Liu, Han-xu;Lin, Wan -ling;Huang, Hui;Liu, Ya-qun;Wei, Ya;Zhao, Yong-qiang;Wang, Yue-qi;Wu, Yan-yan;Chen, Sheng-jun;Li, Lai-hao;Lin, Wan -ling
关键词：Crisp grass carp; Thermal processing; 4D label -free proteomics; Multidimensional data analysis
-
Identification of key physicochemical properties and volatile flavor compounds for the sensory formation of roasted tilapia
作者：Chen, Tianyu;Li, Chunsheng;Huang, Hui;Zhao, Yongqiang;Xiang, Huan;Wang, Di;Feng, Yang;Yang, Shaoling;Chen, Shengjun;Chen, Tianyu
关键词：Roasted tilapia; Sensory property; Texture; Volatile compound; HS-GC-IMS; Correlation network
-
Identification of Key Volatile Compounds in Tilapia during Air Frying Process by Quantitative Gas Chromatography-Ion Mobility Spectrometry
作者：Chen, Tianyu;Xue, Yong;Chen, Tianyu;Li, Chunsheng;Zhao, Yongqiang;Huang, Hui;Feng, Yang;Xiang, Huan;Chen, Shengjun
关键词：tilapia; air frying; oxidation of proteins and lipids; volatile compounds; quantitative GC-IMS; correlation network
-
Integrated Metabolomic and Transcriptomic Analysis Reveal the Production Mechanism of Semicarbazide in Macrobrachium rosenbergii Under Urea Conditions
作者：Li, Jun;Wang, Di;Chen, Shengjun;Gao, Fangfang;Li, Chunsheng;Feng, Yang;Deng, Jianchao;Wang, Di;Chen, Shengjun
关键词：
Macrobrachium rosenbergii ; endogenous semicarbazide; urea; metabolomic; transcriptomic -
Unraveling the antibacterial mechanism of Lactiplantibacillus plantarum MY2 cell-free supernatants against Aeromonas hydrophila ST3 and potential application in raw tuna
作者：Wang, Di;Liu, Yangfan;Chen, Shengjun;Deng, Jianchao;Li, Chunsheng;Pan, Chuang;Wang, Yueqi;Xiang, Huan;Feng, Yang;Yang, Xianqing;Li, Laihao;Wu, Yanyan;Zhao, Yongqiang;Qi, Bo;Wei, Ya;Wang, Di;Chen, Shengjun;Deng, Jianchao;Li, Chunsheng;Pan, Chuang;Wang, Yueqi;Xiang, Huan;Feng, Yang;Yang, Xianqing;Li, Laihao;Wu, Yanyan;Zhao, Yongqiang;Qi, Bo;Wei, Ya;Wang, Di;Chen, Shengjun;Deng, Jianchao;Wang, Di;Chen, Shengjun;Deng, Jianchao;Li, Chunsheng;Pan, Chuang;Wang, Yueqi;Xiang, Huan;Feng, Yang;Yang, Xianqing;Li, Laihao;Wu, Yanyan;Zhao, Yongqiang;Qi, Bo;Wei, Ya;Li, Xupeng;Chen, Shengjun
关键词：Aeromonas hydrophila; Lactiplantibacillus plantarum; Cell free supernatants; Antibacterial; Metabolomics
