文献类型： 外文期刊
作者： Ge, Jun-Qing 1 ; Gao, Guo-Hui 4 ; Xu, Yi-Peng 1 ; Zhang, Chuan-Xi 1 ;
作者机构： 1.Zhejiang Univ, Inst Insect Sci, Hangzhou 310029, Zhejiang, Peoples R China
2.Zhejiang Univ, Minist Agr, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou 310029, Zhejiang, Peoples R China
3.Fujian Acad Agr Sci, Inst Biotechnol, Fuzhou 350003, Peoples R China
4.Wenzhou Med Coll, Sch Life Sci, Wenzhou 325000, Peoples R China
关键词： Bombyx mori nucleopolyhedrovirus;Bm75;P33;Late gene;Nonstructural protein
期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）
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收录情况： SCI
摘要： Open reading frame 75 (Bm-p33) of Bombyx mori nucleopolyhedrovirus (BmNPV) is a homologue of Autographa californica multiple nucleopolyhedrovirus ORF92. The gene is conserved among all baculoviruses that have been completely sequenced to date and is considered to be a baculovirus core set gene. No amino acid mutation was found in Bm-p33 sequences among six BmNPV strains differing in geography, phenotype, or host. The Bm-p33 transcript can be detected as early as 12h post infection (h p.i.) and remains detectable until 96h p.i. The Bm-p33 protein was detected in cell lysates from 18h p.i. through 96h p.i., and no positive band could be detected in budded viruses (BVs) and occlusion-derived viruses (ODVs) by western blot using anti-Bm-p33 serum. Immunofluorescence microscopy indicated that Bm-p33 accumulated in the nuclear membrane and the intranuclear region, especially near the nuclear membrane of the virus-infected cells. Bm75 RNAi significantly decreased the mRNA level. However, no obvious effects on ODV formation and BV production in BmNPV-infected cells could be detected. Bm-p33 is a BmNPV late gene encoding a nonstructural protein which may function mainly in the nucleus of the infected cells.
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