Development, characterization, and cross-species/genera transferability of SSR markers for rubber tree (Hevea brasiliensis)
文献类型: 外文期刊
作者: Yu, Fei 1 ; Wang, Bao-Hua 5 ; Feng, Su-Ping 1 ; Wang, Jing-Yi 1 ; Li, Wei-Guo 2 ; Wu, Yao-Ting 4 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Trop Crop Biotechnol, Minist Agr, Haikou 571101, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Rubber Res Inst, Key Lab Rubber Biol, Minist Agr, Danzhou 571737, Hainan, Peoples R China
3.Hainan Univ, Coll Agr, Key Lab Trop Hort Plant Resources & Genet Improve, Minist Educ, Haikou 570228, Hainan, Peoples R China
4.Qiongzhou Univ, Sanya 572002, Hainan, Peoples R China
5.Nantong Univ, Sch Life Sci, Nantong 226007, Jiangsu, Peoples R China
关键词: polymorphism.;Genomic SSR;Rubber tree (Hevea brasiliensis);Development;Flanking regions;Variation;Internet resource.
期刊名称:PLANT CELL REPORTS ( 影响因子:4.57; 五年影响因子:4.463 )
ISSN:
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收录情况: SCI
摘要: Genomic simple sequence repeat (SSR) markers are particularly valuable in studies of genetic diversity, evolution, genetic linkage map construction, quantitative trait loci tagging, and marker-assisted selection because of their multi-allelic nature, reproducibility, co-dominant inheritance, high abundance, and extensive genome coverage. The traditional methods of SSR marker development, such as genomic-SSR hybrid screening and microsatellite enrichment, have the disadvantages of high cost and complex operation. The selectively amplified microsatellite method is less costly and highly efficient as well as being simple and convenient. In this study, 252 sequences with SSRs were cloned from the rubber tree (Hevea brasiliensis) genome from which 258 SSR loci were obtained. The average repeat number was six. There were only 10 (3.9%) mononucleotide, trinucleotide, and pentanucleotide repeats, whereas the remaining 248 (96.1%) were dinucleotide repeats, including 128 (49.6%) GT/CA repeats, 118 (45.7%) GA/CT repeats, and 2 (0.8%) AT/TA repeats. A total of 126 primer pairs (see ESM) were successfully designed of which 36 primer pairs generated polymorphic products from 12 accessions of the cultivated species, 4 related species, and 3 species of the family Euphorbiaceae. In addition, investigations based on four genomic SSRs (GAR4, ACR22, CTR25, and GTR28) by cloning and sequencing provided evidence for cross-species/genera applicability, and homologous sequences were obtained from the rubber tree and Euphorbiaceae. Further analysis about the variation of the flanking regions of the four markers was carried out.
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