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Plant regeneration via somatic embryogenesis from root explants of Hevea brasiliensis

文献类型: 外文期刊

作者: Zhou, Quan-Nan 1 ; Jiang, Ze-Hai 2 ; Huang, Tian-Dai 1 ; Li, Wei-Guo 1 ; Sun, Ai-Hua 1 ; Dai, Xue-Mei 1 ; Li, Zhe 1 ;

作者机构: 1.Chinese Acad Trop Agr Sci, Rubber Res Inst, State Ctr Rubber Breeding, State Engn & Technol Res Ctr Key Trop Crops, Danzhou 571737, Peoples R China

2.Hainan Univ, Coll Agron, Danzhou 571737, Peoples R China

关键词: Hevea brasiliensis Muell. Arg.;root explants;callus;somatic embryogenesis;regeneration

期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )

ISSN: 1684-5315

年卷期: 2010 年 9 卷 48 期

页码:

收录情况: SCI

摘要: A system for induction of callus and plant regeneration via somatic embryogenesis from root explants of Hevea brasiliensis Muell. Arg. clone Reyan 87-6-62 was evaluated. The influence of plant growth regulators (PGRs) including 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (6-BA) and kinetin (KT) on callus induction of root explants from in vitro plantlets were studied. The results showed that the highest induction frequency of embryogenic callus emerged on Murashige and Skoog (MS) medium supplemented with 1 mg/l KT, 0.2 mg/l 6-BA without 2,4-D. Mean of 4 somatic embryos per embryogenic callus were obtained and approximately 11.8% of them developed into plantlets. The regenerated plantlets were successfully transplanted to sand bed. The plant regeneration system established in this study will facilitate mass propagation and may be applied to culture the roots of high-yielding rubber trees.

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