Production of gynogenetic diploid Polyodon spathula using fertilization with irradiated sperm followed by spontaneous diploidization and distant hybridization caused by heat shock
文献类型： 外文期刊
作者： Zou, Y. C. 1 ; Wei, Q. W. 1 ; Zeng, L. B. 2 ; Li, P. 2 ;
作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China
2.Chinese Acad Fisheries Sci, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist Agr, Yangtze River Fisheries Res Inst, Jinzhou, Hubei, Peoples R China
3.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi, Jiangsu, Peoples R China
4.Univ S Boh
关键词： fertilization;UV irradiation;heat shock;haploidy;gynogenetic diploidy;species inheritance;induce gynogenesis
期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： When paddlefish, Polyodon spathula, eggs were fertilized with UV irradiated (863 mu W cm(-2)) sperm of Amur sturgeon, Acipenser schrenckii, viable gynogenetic diploid progeny with approximately 120 chromosomes occurred due to spontaneous diploidization of the maternal set of chromosomes (SDM). Distant hybridization of P. spathula eggs by A. schrenckii sperm induced inviable gynogenetic haploid progeny with approximately 60 chromosomes. When eggs gynogenetically activated by heterospecific sperm were heat-shocked with 37 degrees C for 2 min at 18 min post-fertilization, a small proportion (7.8%) of viable gynogenetic diploid progeny appeared. The female inheritance of these gynogenetic diploid progeny was verified by chromosome analyses and microsatellite genotyping.
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